CK19和PCNA在肝纤维化大鼠部分肝切除后再生肝中的表达

Abstract

Abstract:

Objective　To detect the expressions of CK19 and PCNA in fibrosis rat regenerating liver after partial hepatectomy (PH) at the different time phase, for evaluating the bile duct regeneration in fibrotic liver.　Methods　Male SD rats were divided into two groups: the control group (42 rats) and the experimental group (42 rats). The rats of experimental group were injected CCl4 to establish the hepatic fibrosis model, and then the two groups was endured PH operation. HE, immunohistochemical and double-label immunofluorescence were used to detect and analyze expressions of CK19 and PCNA in regenerating liver.　Results　The expression of CK19 increased gradually after PH in both groups, however, expression intensity in experimental animals was significantly higher than control ones at the same time point. As well, the expression of PCNA increased gradually after PH in both groups, however, expression level in experimental groups rose slowly, extended and the peak appeared later than the control.　Conclusions　(1) PH process of fibrotic rat liver can stimulate proliferation of OCs and the differentiation to BECs, which mainly originate from compensatory hyperplasia in normal rats regenerating liver. (2) Before PH, liver cells in fibrosis rats have already proliferated, after PH, the effective regenerating cells are lower than the other liver injury.

Key words: Rat, Hepatic fibrosis, Bile duct, CK19, PCNA

CLC Number:

R575

FANG Fang, LIU Jian-Jin, YUE Hua-Jiang, LI Na-Na, LIU Heng-Xin. Expression of CK19 and PCNA in fibrosis rat regenerating liver after partial hepatectomy[J]. Chinese Journal Of Clinical Anatomy, 2012, 30(5): 552-555.

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Expression of CK19 and PCNA in fibrosis rat regenerating liver after partial hepatectomy

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