振动运动对大鼠骨骼肌AMPK-GLUT4糖代谢通路的影响

Abstract

Abstract:

Objective　To study the effects of different vibration training on glycometabolism of rat skeletal muscle, and further explore the suitable vibration training stimulus.　Methods　42 male SD rats were divided randomly into 7 groups according to the vibration frequency (15, 25, 35 Hz) and time (5 min and 15 min) imposed：quiet control group(CC), Low-frequency vibration short group(LS), Low-frequency vibration prolonged group(LL), Medium-frequency vibration short time group(MS), Medium-frequency vibration prolonged time group(ML), High-frequency vibration short time group(HS) and High-frequency vibration prolonged group(HL). Vibration frequency is high frequency (35 Hz), Medium frequency (25 Hz) and low frequency (15 Hz), and training time is short (5 min) and prolonged (15 min), 6 days in one week, the experiment lasted for 8 weeks. After the last training bout (8 weeks), the rats were euthanized, and then the tissue samples were collected. The fasting blood-glucose in blood, muscle glycogen, muscle creatine kinase (CK) and muscle fiber cross-sectional area were analyzed. Adenosine Monophosphate Activated Protein Kinase (AMPK) and Glucose Transporter Four (GLUT4) were analyzed by immunohistochemistry and western blot. Results　(1) Compared with the CC group, in the ML group, the fasting blood-glucose in blood was significantly lower, the muscle fiber cross-sectional area was significantly reduced, the muscle glycogen and muscle creatine kinase (CK) were significantly higher (P<0.05). (2) Compared with the CC group, the expression of ampk-glut4 in each group were significantly increased after vibration training, and the ML group was significantly higher than other experimental group (P<0.05).　Conclusions (1) Vibration training can enhance the glycometabolism of Rat Skeletal Muscle. (2) The suitable vibration training stimulus (25 Hz,15 min) is helpful to increase the glycometabolism of rat skeletal muscle.

Key words: Vibrationtraining, Glycometabolism, Skeletal Muscle, Ampk-glut4

CLC Number:

Q493.4

GONG Xiao-Juan, LIU Chang, JIANG Ding-Wen, MEI Xi-Fan, FANG Shi-Jiang. Effects of vibration training on AMPK-GLUT4 passage of Rat skeletal muscle[J]. Chinese Journal Of Clinical Anatomy, 2013, 31(3): 293-298.

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Effects of vibration training on AMPK-GLUT4 passage of Rat skeletal muscle

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