MTT与HOECHST染色法检测膜联蛋白A7基因沉默对Hela细胞的影响

doi:10.13418/j.issn.1001-165x.2014.01.014

Chinese Journal of Clinical Anatomy ›› 2014, Vol. 32 ›› Issue (1): 57-60.doi: 10.13418/j.issn.1001-165x.2014.01.014

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The effect of Annexin A7 knockdown on Hela cells by MTT and HOECHST stain

CHEN Xue¹, LI Xin3, GAO Fu-lu², ZHANG Jin-ku¹

1.Department of Pathology, Baoding Central Hospital, Baoding 071000, China; 2. Department of Life Science, Hebei Normal University, Shingjiazhuang 050024, China; 3.Department of Histology and Embryology, Chengde Medical College, Chengde 067000, China

Received:2013-08-02 Online:2014-01-25 Published:2014-02-11

Abstract

Abstract:

Objective　To study whether the knockdown of Annexin A7 can affect apoptosis and proliferation of HeLa cells.　Methods　(1)Immunohistochemistry and Western blot were used to identify the expression of Annexin A7 in human cervical carcinoma cell line Hela and to identify the inhibitory efficiency of siRNA interference on the Annexin A7 expression. (2) MTT assay was conducted to detect the impact on the proliferation of HeLa cells after the expression of annexin A7 was inhibited by siRNA. (3) Laser confocal microscope was conducted to detect the impact on the apoptosis of HeLa cells after the expression of Annexin A7 was inhibited by siRNA. 　Results　(1) Annexin A7 was expressed in Hela cells and the Annexin A7 expression of siRNA group was reduced significantly (P<0.05), compared with that of the negative control group and the control group. (2)The cell proliferation of ANXA7siRNA group showed no significant difference compared with that of the negative control group and the control group (P>0.05). (3) The cell apoptotic rate of ANXA7siRNA group was significantly increased (P<0.05) compared with that in the negative control group and the control group. 　Conclusion　The knockdown of Annexin A7 promotes the apoptosis of Hela cells.

Key words: Annexin A7, Hela cells, siRNA

CLC Number:

Q782

CHEN Xue, LI Xin, GAO Fu-Lu, ZHANG Jin-Ku. The effect of Annexin A7 knockdown on Hela cells by MTT and HOECHST stain[J]. Chinese Journal of Clinical Anatomy, 2014, 32(1): 57-60.

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The effect of Annexin A7 knockdown on Hela cells by MTT and HOECHST stain

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