Bmi-1、hTERT在乳腺癌细胞中的表达及与凋亡的关系

doi:10.13418/j.issn.1001-165x.2014.01.015

Abstract

Abstract:

Objective　To study the expression changes of proto-oncogene mi-1 and hTERT, and its relationship with apoptosis and to discuss their roles in the occurrence and development of breast cancer. Methods MTT was used to detect the inhibition of breast cancer cell proliferationand the drug concentrations in the experiment; RT-PCR and Western Blot were used to detect the protein and mRNA expression. TUNEL and Flow cytometry were used to detect the apoptosis situation. Results 5-FU could significantly inhibit the growth of breast cancer cell. The differences of OD value between the groups were statistically significant (P<0.05). Under the intervention of 5-Fu, the mRNA and Western Blot expression levels of Bmi-1 and hTERT gradually decreased, and the apoptosis index increased, which exhibited great statistical significance (P<0.05) compared with the control group. Bmi-1 and hTERT showed a positive correlation (P＜0.05),and their expression had a negative correlation with the apoptosis through the statistical analysis. Conclusion The down-regulation of Bmi-1 and hTERT expression in breast cancer cells can promote the apoptosis.

Key words: Breast cancer, Bmi-1, hTERT, Apoptosis

CLC Number:

R737.9

LIU Chun-Ling, LI Xiao-Chi, YANG Xu, GAO Feng-Lan, DU Hua-Zhen. Bmi -1,hTERT expression in breast cancer cells and its relationship with apoptosis[J]. Chinese Journal of Clinical Anatomy, 2014, 32(1): 61-66.

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Bmi -1,hTERT expression in breast cancer cells and its relationship with apoptosis

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