茶多酚预防慢性酒精中毒对精子损伤的作用及机制研究

doi:10.13418/j.issn.1001-165x.2014.05.022

Abstract

Abstract:

Objective　To explore the preventive role of tea polyphenol (TP) on the chronic alcoholism induced sperm damage and the potential mechanisms.　Methods　Forty male Kunming mice were randomly divided into chronic alcoholism (CA) group, TP low-dosage (TP-L) group, TP high-dosage (TP-H) group and normal control (NC) group. Eight hours after daily alcohol administration, the mice in CA, TP-L and TP-H groups were treated with water, 1% TP or 2% TP respectively. The mice in NC group were administered with water twice a day. 90 days later, sperm suspension was prepared to detect sperm density, activity ratio and malformation ratio; Bcl-2 and Bax expression were observed in testicular tissue via immunohistochemistry.　Results　In CA group, the density, activity ratio of sperm and ratio of Bcl-2 positive cells in testicular tissue were lower, the ratios of sperm malformation and Bax positive cells in testicular tissue were higher than that of NC group. Above changes were reversed in the TP-L and TP-H groups. Moreover, this benign effect showed a positive correlation with dosage.　Conclusion　Tea ployphenol could alleviate alcoholism induced sperm damage. This effect might relate with the Bcl-2 and Bax expression during spermatogenesis.

Key words: Tea polyphenols, Alcoholism, Sperm, Testis, Bcl-2, Bax

CLC Number:

R285.5

SHANG Yin-Juan, WANG Jian-Jun, WANG Xiang-Hai, WEN Jin-Kun, BO Meng-Jie, JIAN Chang-Hui, KANG Wei-Xiang, CHEN Zhuo. Tea polyphenol alleviates the chronic alcoholism induced sperm damage[J]. Chinese Journal of Clinical Anatomy, 2014, 32(5): 604-608.

References

[1] Kurus M, Ugras M, Ates B, et al. Apricot ameliorates alcohol induced testicular damage in rat model
[J].Food Chem Toxicol, 2009,47(10):2666-2672.

[2] Oliva SU, Messias AG, Silva D, et al. Impairment of adult male reproductive function exposed to ethanol since puberty
[J]. Reprod Toxico, 2006, 22(4):599-605.

[3] Lee HY, Naseer MI, Lee SY, et al. Time-dependent effect of ethanol on GnRH and GnRH receptor mRNA expression in hypothalamus and testis of adult and pubertal rats
[J]. Neurosci Lett, 2010,471(1): 25-29.

[4] Giannessi F, Giambelluca MA, Grasso L, et al. Curcumin protects Leydig cells of mice from damage induced by chronic alcohol administration
[J]. Med Sci Monit, 2008,14(11):BR237-242.

[5] Mukhtar H, Ahmad N. Tea polyphenols: prevention of cancer and optimizing health
[J]. Am J Clin Nutr, 2000, 71(6 Suppl):1698S-1702S.

[6] Darvesh AS, Bishayee A. Chemopreventive and therapeutic potential of tea polyphenols in hepatocellular cancer
[J]. Nutr Cancer, 2013, 65(3):329-344.

[7] Hugel HM, Jackson N. Redox chemistry of green tea polyphenols: therapeutic benefits in neurodegenerative diseases
[J]. Mini Rev Med Chem, 2012, 12(5):380-387.

[8] Heinrich U, Moore CE, De Spirt S. et al. Green tea polyphenols provide photoprotection, increase microcirculation, and modulate skin properties of women
[J]. J Nutr, 2011,141(6): 1202-1208.

[9] Shen CL, Yeh JK, Samathanam C, et al. Green tea polyphenols attenuate deterioration of bone microarchitecture in female rats with systemic chronic inflammation
[J]. Osteoporos Int, 2011, 22(1):327-337.

[10] 王文祥, 张文昌, 廖惠珍. 茶多酚对镉致大鼠精子损伤的保护作用
[J].福建医科大学学报, 2011, 45(5): 319-322, 326.

[11] 江莉, 李慕军, 李娜, 等. 密度梯度离心加上游法处理对精子形态学的影响
[J].广西医学, 2012, 34 (1):38-40.

[12] Wallock-Montelius LM, Villanueva JA, Chapin RE, et al. Chronic ethanol perturbs testicular folate metabolism and dietary folate deficiency reduces sex hormone levels in the Yucatan micropig
[J].Biol Reprod, 2007, 76(3):455-465.

[13] Vergara SP, Lizama C, Brouwer-Visser J, et al. Expression of BCL-2 family genes in germ cells undergoing apoptosis during the first wave of spermatogenesis in the rat
[J]. Andrologia, 2011, 43(4):242-247.

[14] Meehan T, Loveland KL, de Kretser D, et al. Developmental regulation of the bcl-2 family during spermatogenesis: insights into the sterility of bcl-w-/- male mice
[J]. Cell Death Differ, 2001, 8(3):225-233.

[15] Yamamoto CM, Hikim AP, Lue Y, et al. Impairment of spermatogenesis in transgenic mice with selective overexpression of Bcl-2 in the somatic cells of the testis
[J]. J Androl, 2001, 22(6):981-991.

[16] Yuan H, Deng Y, Yuan L, et al. Gynostemma pentaphyllum protects mouse male germ cells against apoptosis caused by zearalenone via Bax and Bcl-2 regulation
[J]. Toxicol Mech Methods, 2010, 20(3):153-158.

[17] Albrecht H, Tschopp J, Jongeneel CV. Bcl-2 protects from oxidative damage and apoptotic cell death without interfering with activation of NF-kappa B by TNF
[J]. FEBS Lett, 1994, 351(1):45-48.

Tea polyphenol alleviates the chronic alcoholism induced sperm damage

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	Meng Xingqi, Deng Jing, Peng Lixuan, Zhang Yuan, Lin Yi, Zhou Xiaobing, Cao Wenyu, Li Suyun. Effects of streptozotocin on spermatogenic function and testicular m6A methylase expression in diabetic mice [J]. Chinese Journal of Clinical Anatomy, 2023, 41(2): 182-186.
[2]	Pang Ting, Tang Qiulian, Chen Yong, Wei Jiangcun, Liang Cao, Wang Zhiqiang, He Huan, Zeng Chao. Mechanism of herb-pair Trachelospermum jasminoides and Achyranthes bidentata treating Arthritis based on network pharmacology [J]. Chinese Journal of Clinical Anatomy, 2022, 40(5): 569-574.
[3]	SUN Li, HUANG Gui, GONG Xiang-jun , OU Jian-ping. Digital holographic microscopy (DHM) and its application in monitoring three-dimensional dynamics of human spermatozoa [J]. Chinese Journal of Clinical Anatomy, 2020, 38(4): 476-480.
[4]	ZENG Peng, QI Rui, WANG Jin, SUN Xiao, DAI You-guo, ZHANG Peng-fei, YANG Li, GUO Ze-yun. Protective effects of L-carnitine on testicular injury induced by cyclophosphamide in rats through β-catenin signaling [J]. Chinese Journal of Clinical Anatomy, 2020, 38(3): 283-288.
[5]	SU Qing, CAO Li, PENG Zhuo, LI Qi, YI Liu-xiang, GUO Xian-xian, LIU Fang-ting, SUN Guo-ying. Spermidine alleviates acute lung injury induced by lipopolysaccharide in mice [J]. Chinese Journal of Clinical Anatomy, 2020, 38(1): 62-66.
[6]	LI Qing, HU Shao-fei, WANG Li-hua, HUANG Yi, DENG Zhu-xiu, ZHU Mao-ling. Impact of sperm parameters on the outcome of intrauterine insemination cycles [J]. Chinese Journal of Clinical Anatomy, 2019, 37(4): 436-441.
[7]	MAO Qiang, YANG Tao, Zhou Ke-song, QI Su. Comparison of ultrasonographic guided fine needle aspiration and core needle biopsy for testicular tissue [J]. Chinese Journal Of Clinical Anatomy, 2018, 36(1): 98-100.
[8]	MAO Yi, CUI Xin-gang. Clinical significance of imaging observation on spinous process and interspinous space anatomy of subaxial cervical spine [J]. Chinese Journal Of Clinical Anatomy, 2017, 35(6): 637-640.
[9]	HU Xiao-fang1,2, QIU Ren-jie4, YU Lei2, LIU Mao-sheng1, YANG Lin1, WANG Guo-bao2,3. Synthesis nano-carrier SWCNT to deliver Bcl-2 siRNA and induction of U251 cell apoptosis [J]. Chinese Journal Of Clinical Anatomy, 2017, 35(5): 521-525.
[10]	TANG Yin-juan，WANG Jian-jun,WANG Xiang-hai,PAN Meng-jie,CHEN Zhuo,XIAO Ling-long, LIU Jia-hua, CAI Wei-jun. The research of tea polyphenols for liver failure due to chronic alcoholic liver damage [J]. Chinese Journal Of Clinical Anatomy, 2016, 34(2): 198-201.
[11]	SONG Hai-Yan, GAO Zhi-Chao, DENG Xiao-Hui, LIAN Hui, LIN Ming-Xin, FU Sheng-Qi. The expression of Bax and Bcl-2 in cultured neonatal rat cortical neurons and the role of L-NAME intervention [J]. Chinese Journal of Clinical Anatomy, 2014, 32(5): 595-598.
[12]	TAN Shuo-Guan, LIANG Wei-Bei, BO Bin. The modified radical orchiectomy of clinical stage-Ⅰ seminoma of the testis [J]. Chinese Journal Of Clinical Anatomy, 2013, 31(6): 718-720.
[13]	FANG Zhi-Qi, TUN Gang, WANG He-Ban, CHEN Xiao-Yu. Correlation of apoptosis genes of p53，bcl-2 and bax in tissue of prostate [J]. Chinese Journal Of Clinical Anatomy, 2013, 31(5): 560-563.
[14]	SONG Hai-Yan, LI Na-Na, FU Sheng-Qi, LIN Ming-Xin. Expression of Bax and NOS in neurons received excitatory neurotoxicity induced by NMDA [J]. Chinese Journal Of Clinical Anatomy, 2012, 30(6): 675-677.
[15]	LIN Yong-sui, WANG Wan-ming, ZHENG He-ping, ZHANG Fa-hui, WANG Chun. Anatomy and clinical significance on blood supply of the subaxial cervical vertebrae [J]. Chinese Journal Of Clinical Anatomy, 2012, 30(3): 247-250.