MCSCs-exosomes对成年大鼠心肌细胞缺氧损伤的干预作用

doi:10.13418/j.issn.1001-165x.2014

Abstract

Abstract:

Objective　To observe the apoptosis of injured adult rats myocardial cells induced by ischemia and hypoxia in vitro and the biological functions of the exosomes from marrow-derived cardiac stem cells (MCSCs-exosomes)．Methods　Cultured adult rats myocardial cells were divided into the control group，the ischemia-hypoxia group and the MCSCs-exosomes pretreated group． In vitro，we counted the cells and detected the expression of Bcl-2，bax by Western Blotting．The cell cycles and apoptosis were measured by flow cytometry． Results　Compared with the control group，the model group showed lower cell counts(P<0.01) and higher apoptosis ratio． We also observed that the expression of Bcl-2 / bax was decreased(P<0.01) and the cell cycle was blocked at S phase．Compared with the ischemia-hypoxia group，MCSCs-exosomes pretreated groups showed apparent tolerance to ischemic and hypoxic injury in vitro，which manifested a higher cell viability，lower apoptosis ratio，increased expression of Bcl-2/bax and relief of the block of S phase( all P<0.05)． Conclusions Ischemia and hypoxia could cause the apoptosis of cells in vitro.MCSCs-exosomes exhibited anti-apoptosis ability in vitro．All these may provide experimental evidences for the mechanisms of MCSCs-exosomes in therapy of tissue injury．

Key words: Marrow-derived cardiac stem cells, Exosomes, Adult rats myocardial cells, Hypoxia, Apoptosis

CLC Number:

Q813.1

CUI Xiao-Jun, ZHANG Jian-Kai, ZHANG Zhi, ZHOU Li-Na, GUO Jin-Hua, WANG Xiao-Gong, MA Ying-La. Effect of the exosomes from marrow-derived cardiac stem cells on apoptosis in the ischemia and hypoxia injured myocardial cells from adult rats[J]. Chinese Journal Of Clinical Anatomy, 2014, 32(6): 685-689.

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Effect of the exosomes from marrow-derived cardiac stem cells on apoptosis in the ischemia and hypoxia injured myocardial cells from adult rats

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