不同剂量吗啡对C57小鼠成瘾行为学的影响

doi:10.13418/j.issn.1001-165x.2014

Abstract

Abstract:

Objective　To observe the conditioned place preference (CPP) changes in mice after treated with different doses of morphine, and provide reference for the further study of morphine addiction. Method 　3-month-old male C57 mice were injected intraperitoneally with 0 mg/kg, 5 mg/kg, 10 mg/kg, 15 mg/kg and 20 mg/kg of morphine, CPP and Straub tail reaction analysis were carried out. Result The Straub tail reaction analysis showed that: compared with control group, mice in morphine 10 mg/kg, 15 mg/kg, and 20 mg/kg groups demonstrated longer Straub tail reaction time (P< 0.05). The CPP analysis also showed that, compared with the control group mice，15mg/kg and 20mg/kg morphine reversed the natural tendency to black box of mice, which spent more time in the white box (P< 0.001). Meanwhile, the time spend in the white box was also statistically longer in the 10 mg/kg morphine group compared with the control group (P=0.049<0.05).　Conclusion 　15 mg/kg morphine can produce a significant adaptive change in CPP test in mice, which is an ideal dosage to construct morphine addiction model in mice.

Key words: C57 mice, Morphine, Dosage, Conditioned place preference, Straub tail reaction

CLC Number:

R331

XIE Min-Juan, LI Juan, ZHANG Lu. Effects of different dosages of morphine on conditioned place preference in male C57 mice[J]. Chinese Journal Of Clinical Anatomy, 2014, 32(6): 708-710.

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Effects of different dosages of morphine on conditioned place preference in male C57 mice

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