一氧化碳中毒迟发性脑病小鼠脑HO-1表达变化及其与细胞凋亡的关系

doi:10.13418/j.issn.1001-165x.2014

Abstract

Abstract:

Objective　To study the expression of the heme oxygenase-1 in the brain of mice at different time points after carbon monoxide poisoning. To detect neuron apoptosis and related gene expression in the brain of mice at different time points after carbon monoxide poisoning. To investigate the relationship between changes in heme oxygenase-1 and apoptosis of brain cells. 　Methods　Carbon monoxide poisoning delayed encephalopathy model by using intraperitoneal injection of carbon monoxide. Application The expression of HO-1 protein levels was observed By Western blotting analysis. Flow cytometry was used to detect apoptotic cells. Application immunohistochemistry SABC observed Bcl-2 and Bax expression at the protein level.　Results　Expression of HO-1 protein is less in the air Control group by Western blotting；expression increased at 1d (1.14±0.43) (P<0.01), reaching a peak at 3d (2.91±1.07) (P<0.01) in CO poisoning group；Expression of HO-1 protein in poisoning group was still higher than in that in the control group at 21d (P<0.01). Apoptosis: the percentage of apoptotic cells increased in CO poisoning 12h group (21.95±24) (P<0.01), reaching the peak at 5d (34.27±4.19) (P< 0.01). The percentage of apoptotic cells was still high at 21d (9.25±1.16). Protein related: Bax expression increased after the poisoning, reaching the peak at 3d (34.5±0.58)( P<0.01). Bcl-2 expression reduced to a minimum at 3d (12.5±3.69) (P<0.01). The ratio of Bax/Bcl-2 reached maximum at 3d.　Conclusion　Expression of heme oxygenase-1 protein and Apoptotic cells were significantly increased in the hippocampus after delayed encephalopathy in mouse of carbon monoxide poisoning. The changes of apoptotic cells were consistent with heme oxygenase-1 protein expression. The upregulated expression of heme oxygenase-1 protein plays a crucial role in the pathogenesis of delayed encephalopathy after CO poisoning.

Key words: Carbon monoxide poisoning, Hemeoxygenase-1, Neural apoptosis, Apoptosis gene, Mice

CLC Number:

R318

DIAO Lin-Yan, LI Fang, WANG Su-Beng, XU Jian. Changes of heme oxygenase-1 and apoptosis as well as the relationship between them following carbon monoxide poisoning delayed encephalopathy in mice[J]. Chinese Journal Of Clinical Anatomy, 2014, 32(6): 711-715.

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Changes of heme oxygenase-1 and apoptosis as well as the relationship between them following carbon monoxide poisoning delayed encephalopathy in mice

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