Abstract:

Objective    To study the three-dimensional porous scaffolds of polylactic acid/nano- hydroxyapatite (PLLA/n-HA) in vitro biocompatibility, and to explore its suitability ascell culture materials and bone tissue engineering scaffolds.   Methods    The rat osteoblasts were seeded on PLLA/n-HA composite scaffolds after in vitro co-culture, CCK-8 assay was used to determine the rat osteoblast proliferation activity, inverted fluorescence microscope; scanning electron microscope were used to observe the PLLA/n- HA composite scaffold surface and the cell adhesion conditon within the pores.   Results    CCK-8 assay showed that the experimental group was not significantly different in proliferation of composite scaffolds cells with the control group (P>0.05); electron microscopy showed a large number of cell growth and adhesion in the composite scaffold surface and pores; and with the increase of culture time, the number of cells on  material surface grew exponentially.   Conclusion    The three-dimensional porous scaffolds PLLA/n-HA have good biocompatibility and is expected to become a good performance of bone tissue engineering scaffolds.

Key words:  Three-dimensional porous, PLLA, n-HA, Rat osteoblasts