不同空间排列静电纺丝对RSC96细胞表达NGF及Laminin的研究

doi:10.13418/j.issn.1001-165x.2015

Chinese Journal Of Clinical Anatomy ›› 2015, Vol. 33 ›› Issue (1): 60-63.doi: 10.13418/j.issn.1001-165x.2015

Previous Articles Next Articles

The influence of topography of electrospun fibrous scaffolds on NGF and Laminin expressions of RSC96 cells

CAO Xin ^1,2, ZHAO Li², ZHANG Xian-zhuo², QIN Jian-qiang ^2,3， LI Hong ⁴， WU Gang⁵

1.Department of Neurology, Affiliated Xiao Lan People Hospital, Southern Medical University, zhongshan, 528415, China; 2. Department of Anatomy, Southern Medical University, Guangzhou 510515, China; 3. Department of Chinese Medicine, Macau university of science and technology, Macau 999078; 4 .College of Science and Engineering,JINAN university, Guangzhou 510632, China; 5.South China University of Technology, Guangzhou 510641, China

Received:2014-04-27 Online:2015-01-25 Published:2015-02-12

Abstract

Abstract:

Objective To study the expression of NGF and Laminin in RSC96 cells under different topography of eletrospun fibrous scaffolds. Methods According to different culture conditions, cultured RSC96 were divided into three groups, group A(aligned), group B(random) and group C(film), and all the three groups were cultured for three days. The total RNA of cells was extracted by Trizol method. Reverse transcriptase Oligo (dT) was used to synthesize cDNA. Then RNA was expanded by designed primer and used for q-PCR to detect the expression of NGF and Laminin. Results NGF mRNA expression of RSC96 was lower in group A , compared to group B and C (P＜0.05). There was statistical differences between group B and group C (P＜0.05). The laminin mRNA was expressed in all groups, but an decrease from group A to group C could be detected (P＜0.05). Conclusions The different expression of NGF and Laminin of RSC96 cells was induced by different topography of electrospun fibers and RSC96 cells may be directed towards a more mature state by aligned electrospun fibers.

Key words: RSC96 cells, NGF, Laminin, Electrospun fibers

CLC Number:

R322.8

CAO Xin, DIAO Li, ZHANG Xian-Zuo, QIN Jian-Jiang, LI Gong, TUN Gang. The influence of topography of electrospun fibrous scaffolds on NGF and Laminin expressions of RSC96 cells[J]. Chinese Journal Of Clinical Anatomy, 2015, 33(1): 60-63.

References

[1] Ndubaku U, de Bellard ME. Glial cells: old cells with new twists
[J]. Acta Histochem, 2008, 110(3): 182-195.

[2] Bunge RP. The role of the Schwann cell in trophic support and regeneration
[J]. J Neurol, 1994, 242(Suppl 1): S19-21.

[3] Griffin JW, Thompson WJ. Biology and pathology of nonmyelinating Schwann cells
[J]. Glia, 2008, 56(14): 1518-1531.

[4] Woodhoo A, Sommer L. Development of the schwann cell lineage: from the neural crest to the myelianted nerve
[J]. Glia, 2008, 56(14):1481-1490.

[5] Jessen KR, Mirsky R. The origin and development of glial cells in peripheral nerves
[J]. Nat Rev Neurosci, 2005, 6(9):671-682.

[6] Nave KA, Trapp BD. Axon-glial signaling and the glial support of axon function
[J]. Annu Rev Neurosci, 2008, 31:535-561.

[7] Hai M, Muja N, Devries G H, et al. Comparative analysis of Schwann cell lines as model systems for myelin gene transcription studies
[J]. J Neurosci Res, 2002, 69(4):497-508.

[8] Mitchel JA, Hoffman-Kim D. Cellular scale anisotropic topography guides schwann cell motility
[J]. PLoS ONE, 2011, 6(9): e24316.

[9] Gu Y, Ji Y. The influence of substrate stiffness on the behavior and functions of Schwann cells in culture
[J]. Biomaterials, 2012, 33(28):6672-6681.

[10] Chew SY, Mi R. The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation
[J]. Biomaterials, 2008, 29(6):653–661.

[11] Valmikinathan CM, Hoffman J. Impact of scaffold micro and macro architecture on Schwann cell proliferation under dynamic conditions in a rotating wall vessel bioreactor
[J]. Mater Sci Eng C Mater Biol Appl, 2011, 31(1):22-29.

[12] EM Johnson Jr, Taniuchi M. Expression and possible function of nerve growth factor receptors on Schwann cells
[J]. Trends Neurosci, 1988, 11(7):299-304.

[13] Cornbrooks CJ, Carey DJ, McDonald JA, et al. In vivo and in vitro observations on laminin production by schwann cells
[J]. Proc Natl Acad Sci U S A, 1983, 80(12):3850-3854.

[14] Chen ZL, Strickland S. Laminin γ1 is critical for schwann cell differentiation, axon myelination, and regeneration in the peripheral nerve
[J]. J Cell Biol, 2003,163(4):889-899.

[15] Feltri ML, Wrabetz L. Laminins and their receptors in Schwann cells and hereditary neuropathies
[J]. J Peripher Nerv Syst, 2005, 10(2):128-143.

[16] Previtali SC, Nodari A. Expressions of laminin receptors in schwann cell differentiation: evidence for distinct roles
[J]. J Neurosci, 2003, 23(13):5520-5530.

[17] Jessen KR, Mirsky R. Schwann cell precursors and their development
[J]. Glia, 1991, 4(2):185-194.

[18] Palm SL, Furcht LT. Production of laminin and fibronectin by schwann cells: cell-protein interactions in vitro and protein localization in peripheral nerve in vivo
[J]. J Cell Biol, 1983, 96(5):1218-1226.

[19] Ogata T, Yamamoto S, Nakamura K, et al. Signaling axis in Schwann cell proliferation and differentiation
[J]. Mol Neurobiol, 2006, 33(1):51-61.

The influence of topography of electrospun fibrous scaffolds on NGF and Laminin expressions of RSC96 cells

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Metrics

Comments

Recommended 0

[1]	KAN Shi-lian,LI Gui-shi,ZHAN Hai-hua,ZHANG Jian-bing,MA Ning，LI Ming-xin，ZHAO Jia-guo. Changes of motor neurons in spinal cord anterior horn after peripheral nerve graft repair in rat [J]. Chinese Journal Of Clinical Anatomy, 2016, 34(5): 534-539.
[2]	DIAO Li, CAO Xin, OU Yang-Jun, TU Lei, ZHANG Xian-Zuo, LIU Re-Yan, XUE Xu-Kai, LEI Zhi-Xian, QIN Jian-Jiang. The effects of macrophage in different activation states on NGF and Laminin expressions of RSC96 cells [J]. Chinese Journal Of Clinical Anatomy, 2013, 31(3): 321-324.