Abstract:

Objective   To evaluate the effect of different concentrations of 17β-estradiol（17β-E2）on adipocytes lipid droplet growth and CIDEc, Plin1 mRNA expression in vitro.    Methods    Human primary adipose tissue-derived stem cells(hASCs) were isolated from liposuction aspirates of three patients and cultured, passaged and  identified the cell surface markers. hASCs were induced differentiated at 0 mol/L(17β-E2 free DMEM/F12),10-8 mol/L, 10-7 mol/L and 10-6 mol/L 17β-E2 and ICI182780 was added to culture human primary preadipocytes. The expression of CIDEc and Plin1 mRNA was detected by RT-PCR after staining with oil red O.  Result   Different concentrations of 17β-E2 suppressed CIDEc mRNA and Plin1 mRNA expression in human primary preadipocytes. The expression peak of CIDEc and Plin1 mRNA occurred at 7 and 4 days.  At 10-6 mo/L 17β-E2，the expression of CIDEc and Plin1 mRNA were the lowest. Andwith the increase in 17β-E2 concentration，the expression of CIDEc and Plin1 mRNA was in decline.  Conclusion   17β-E2 reduces CIDEc and Plin1 mRNA expression in adipocytes，in which inhibited CIDEc and Plin1 expression may contribute to the negative effect of 17β-E2 on lipid droplets.

Key words: 17β-E2, Lipid droplet, Primary human adipocyte, Adiposed differentiation, CIDEc, Perilipin1