改良人颅咽管瘤原代细胞培养方法

doi:10.13418/j.issn.1001-165x.2015.03.021

Abstract

Abstract:

Objective Using new method to culture the craniopharyngioma cells in order to provide the foundation of clinical and basic researches on cell level. Methods Using new method to culture, purify and pass two pathological patterns of craniopharyngioma cells. The cells were identified by morphology under microscope, immunohistochemistry and proliferation assay. Results The craniopharyngioma cells cultured by the new method were seen around the tumor piece with better state and greater numbers than the cells cultured by the traditional method. These two kinds of cells were determined to be the craniopharyngioma cells. The proliferation assay showed that the cells were still proliferating and dividing in vitro. Conclusions Using new method which was simple and cheap to culture the craniopharyngioma cells could harvest better cells in state and greater in numbers. Though this method could not solve all the problems about cell culture of craniopharyngioma, it could still provide the foundation of clinical and basic researches about tumor biological characteristics on the cell level by establishment of a stable cell line.

Key words: Craniopharyngioma, Primary culture, Cell line

References

[1] Song-Tao Q, Xiao-Rong Y, Jun P, et al. Does the calcification of adamantinomatous craniopharyngioma resemble the calcium deposition of osteogenesis/odontogenesis?[J]. Histopathology,2014,64(3):336-347.
[2] Qi ST, Zhou J, Pan J, et al. Epithelial–mesenchymal transition and clinicopathological correlation in craniopharyngioma[J]. Histopa thology, 2012, 61(4):711-725.
[3] 周杰，漆松涛，潘军，等. 颅咽管瘤组织炎性反应与临床病理和肿瘤预后相关性分析[J]. 中华神经外科杂志, 2013, 29(5):461-464.
[4] 周杰，漆松涛，陈礼刚，等. 不同炎症等级性釉质上皮型颅咽管瘤组织中炎症细胞因子的表达[J]. 中华医学杂志, 2013, 93(31):2499-2501.
[5] Qi S, Huang G, Pan J, et al. Involvement of osteopontin as a core protein in craniopharyngioma calcification formation[J]. J Neurooncol, 2010, 98(1):21-30.
[6] Zada G, Lin N, Ojerholm E, et al. Craniopharyngioma and other cystic epithelial lesions of the sellar region: a review of clinical, imaging, and histopathological relationships[J]. Neurosurg Focus, 2010, 28(4):E4.
[7] 漆松涛，骆实，张喜安，等. 颅咽管瘤侵袭第三脑室的方式对手术切除的影响[J]. 中华神经医学杂志, 2009, 8(6):588-591.
[8] 徐建国，刘亮，饶正西，等. 颅咽管瘤细胞系的初步建立[J]. 四川大学学报: 医学版, 2011,42(3):417-421.
[9] 刘亮，郑晓梅，游潮，等. 一种新的人脑颅咽管瘤细胞系的培养方法[J]. 西部医学, 2013, 25(6):807-810.
[10] Qi S, Lu Y, Pan J, et al. Anatomic relations of the arachnoidea around the pituitary stalk: relevance for surgical removal of craniopharyngiomas[J]. Acta Neurochir (Wien), 2011,153(4):785-796.
[11] Pan J, Qi S, Lu Y, et al. Intraventricular craniopharyngioma: morphological analysis and outcome evaluation of 17 cases[J]. Acta Neurochir (Wien), 2011,153(4):773-784.
[12] Honegger J, Renner C, Fahlbusch R, et al. Progesterone receptor gene expression in craniopharyngiomas and evidence for biological activity[J]. Neurosurgery, 1997, 41(6):1359-1364.
[13] Ulfarsson E, Karström A, Yin S, et al. Expression and growth dependency of the insulin-like growth factor I receptor in craniopharyngioma cells: a novel therapeutic approach[J]. Clin Cancer Res,2005,11(13):4674-4680.
[14] Holsken A, Buchfelder M, Fahlbusch R, et al. Tumour cell migration in adamantinomatous craniopharyngiomas is promoted by activated Wnt-signalling[J]. Acta Neuropathol, 2010,119(5):631-639.
[15] 江荣才，刘宗惠. 颅咽管瘤原代培养和博莱霉素的体外抑瘤实验[J]. 中华神经外科杂志, 2001, 17(1):29-31.
[16] 周良学，游潮. 颅咽管瘤基础研究进展[J]. 中国临床神经外科杂志, 2009, 14(3):188-192.
[17] Li Q, You C, Zhou L, et al. All-trans retinoic acid inhibits craniopharyngioma cell growth: study on an explant cell model[J]. J Neurooncol, 2013,112(3):355-364.
[18] 周杰，漆松涛，潘军，等. 人颅咽管瘤裸鼠皮下移植瘤模型的初步建立[J]. 重庆医学, 2012, 41(5):455-457.
[19] Li Q, You C, Liu L, et al. Craniopharyngioma cell growth is promoted by growth hormone (GH) and is inhibited by tamoxifen: involvement of growth hormone receptor (GHR) and IGF-1 receptor (IGF-1R)[J]. J Clin Neurosci, 2013, 20(1):153-157.
[20] Xu J, Liu L, Zheng X, et al. Expression and inhibition of ADAMDEC1 in craniopharyngioma cells[J]. Neurol Res, 2012, 34(7):701-706.

The improved primary cell culture method of craniopharyngioma

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 4

Metrics

Comments

Recommended 0

[1]	Cai Meiqin, Luo Lun, He Haiyong, Zheng Wenhan, Zhang Baoyu, Li Wensheng, Guo Ying. Application of neuroendoscopy in extended transnasal approach for craniopharyngiomas surgery [J]. Chinese Journal of Clinical Anatomy, 2021, 39(4): 469-472.
[2]	LIU Fang, MAO Zhi-rong, DENG Li, CHEN Bo, YUAN Qiong-lan, GAO Xiao-qing. Neuroprotection of GDNF gene-modified NSCs on temporary ischemic stroke in rats [J]. Chinese Journal of Clinical Anatomy, 2020, 38(4): 408-413.
[3]	DENG Zhao-jian, JIANG Qi-jun, GAN Zheng-kai. Clinical application and effect of microsurgery through eyebrow arch for the treatment of craniopharyngioma [J]. Chinese Journal of Clinical Anatomy, 2019, 37(6): 704-707.
[4]	ZHAO Yang, WAN Yin-xu, CHE Ji-zhong, XU Yan-kai, LI Qing-yuan. Overexpression of miR-128-3p inhibits cell invasion, migration and epithelial-mesenchymal transition by targeting MAPK1 in bladder cancer cell line 5637 [J]. Chinese Journal of Clinical Anatomy, 2019, 37(5): 534-541.