诱导型一氧化氮合酶对骨骼肌损伤再生的影响

doi:10.13418/j.issn.1001-165x.2015.05.013

Abstract

Abstract:

Objective　To observe the morphological changes and iNOS expression at cellular damaged zone during the wound healing of denervated muscle; To determine the affection of the expression of iNOS induced by supernatant of macrophages.　Methods　The damaged denervated muscle, three days after the damaged muscle model of rat was established, was harvested for H.E and immunohistochemical staining of iNOS. The cultured C2C12 cells were treated with supernatant of macrophages. The treated C2C12 cells were analyzed using RT-PCR method.　Results　New capillaries near the damaged muscle and high expression of iNOS could be observed. The expression of iNOS was different between the damaged and undamaged zones in muscle cells. The supernatant of macrophages could induce the expression of iNOS and MyoD genes. Conclusion　The high expression of iNOS can accelerate the regeneration of denervated muscle during wound healing.

Key words: Skeletal muscle, Wound healing, iNOS, Angiogenesis

References

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Role of iNOS during wound healing of skeletal muscle

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