大鼠纤维化心肌组织中神经钙黏附素蛋白的表达变化

doi:10.13418/j.issn.1001-165x.2016.01.020

Chinese Journal Of Clinical Anatomy ›› 2016, Vol. 34 ›› Issue (1): 78-82.doi: 10.13418/j.issn.1001-165x.2016.01.020

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Influence of neural cadherin protein expression on myocardial fibrosis in rats

MU Ling-min¹, YAN Xiao-xiao¹, ZHENG Hui², ZHANG Guang-mou³ , GUO ZHi-kun⁴

1.Morphologial Laboratory of Xinxiang Medical College, 2. Affiliated Hospital of Third of Xinxiang Medical College, 3.Cell Engineering Department of Life Science and Technique College, Xinxiang Medical College； 4. The key Laboratory for Tissue Regeneration of Henan Universities, Xinxiang 453003, China

Received:2015-06-06 Online:2016-01-25 Published:2016-01-28

Abstract

Abstract:

Objective　To investigate the expression of neural cadherin (N-cadherin) on isoproterenol induced myocardial fibrosis in rat, and provide morphological data for the investigation of the cardiac hypertrophy signal transduction mechanism and reversal of cardiac hypertrophy．Methods The rat models of myocardial hypertrophy were prepared by subcutaneous injection with isoprenaline (ISO) to healthy adult SD rats. The pathological changes of the myocardial collagen fiber were observed conventional paraffin section and Masson staining. The expression and distribution of N-cadherin was detected by immunohistoehemistry and immunofluorescence staining．Reverse transcription polymerase chain reaction was used to determine mRNA expression levels of N-cadherin in the myocardial tissues. Image analysis software was used to conduct quantitative analysis. Results Compared with the control group, the positive expression of N-cadherin protein in the experimental group had no significant difference. Furthermore, mRNA expression of N-cadherin in the myocardial tissues of rats was consistent with the immunohistoehemistry results. Conclusion The N-cadherin protein may be necessary for maintaining the structure and physiological function of myocardium.

Key words: Neural cadherin, Immunohistohemistry, Immunofluorescence staining, Myocardial fibrosis

References

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Influence of neural cadherin protein expression on myocardial fibrosis in rats

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