麝香保心丸通过抑制p38 MAPK和NF-κB信号传导通路表达对抗高糖诱导的心肌细胞损伤

doi:10.13418/j.issn.1001-165x.2016.05.009

Abstract

Abstract:

Objective　To explore whether Shexiang Baoxin Pill（SXBXP/SBP）protects H9c2 cardiac cells against high glucose（HG）-induced injury by inhibition of p38 MAPK and NF-κB pathway．Methods　H9c2 cardiac cells were treated with 35 mmol/L glucose（HG）for 24h to establish a model of HG-induced injury；Cell counter kit-8（CCK-8）was used to measure cell viability；Apoptotic cells were tested by Hoechst 33258 nuclear staining followed by fluorescence imaging；Intracellular levels of reactive oxygen species（ROS）were detected by 2’，7’-dichlorfluorescein-diacetate（DCFH-DA）staining，followed by fluorescence imaging; Mitochondrial membrane potential （MMP）was measured by a fluorescent dye，rhodamine 123（Rh123），followed by fluorescence imaging；The expression levels of p38 MAPK and NF-κB p65 protein were detected by Western blot assay.　Result　Treatment of H9c2 cardiac cells with HG for 24h signally induced injuries，proved by a decrease in cell viability，an increase in amount of apoptotic cells and intracellular ROS production，as well as a loss of MMP；HG strengthened the expression of phosphorylated（p）-p38 MAPK and p-NF-κB p65; Pretreatment with SBP notably inhibited the up-regulation of expression of p-p38 MAPK and p-NF-κB p65 induced by HG; Pretreatment with SBP or SB203580，an inhibitor of p38 pathway or PDTC, an inhibitor of NF-κB pathway，attenuated the above HG-induced injuries，including cytotoxicity，apoptosis，increase in ROS production and a loss of MMP，in H9c2 cardiac cells.　Conclusion　SXBXP（SBP） may protect H9c2 cardiac cells against the HG-induced injuries by inhibiting the p38 MAPK and NF-κB pathway．

Key words: Shexiang Baoxin Pill, High glucose, p38 MAPK, NF-κB, Cardiomyocyte

YANG Yi-ying, CHEN Wei-yan, SUN Xiu-ting, WANG Xiang, LI Zheng-xun, LIANG Mei-ling, SHI Hui, YANG Zhi-sheng, ZENG Wu-tao. Shexiang baoxin pill protects cardiomyocytes against high glucose-induced injury by inhibiting the p38 MAPK and NF-κB pathway[J]. Chinese Journal Of Clinical Anatomy, 2016, 34(5): 517-522.

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Shexiang baoxin pill protects cardiomyocytes against high glucose-induced injury by inhibiting the p38 MAPK and NF-κB pathway

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