BMP-2转染BMSCs复合镁合金棒修复兔股骨头坏死的实验研究

doi:10.13418/j.issn.1001-165x.2016.05.011

Abstract

Abstract:

Objective 　To investigate the effect of BMSCs transfected BMP-2 composite with magnesium alloy rod in the repair of the femoral head necrosis. Methods Rabbit BMSCs was isolated, cultured, and successfully transfected pcDNA3.1-BMP-2 plasmid by electroporation. 40 rabbit femoral head necrosis was established by liquid nitrogen freezing method, and then were randomly divided into 4 groups of 10 animals each: the magnesium rod /BMSCs group, the magnesium rod group, the BMSCs group, and the blank group. The bio-magnesium alloy rod imported the BMSCs-BMP-2 plasmid pcDNA3.1. Repair effect was assessed post-operatively at 6 weeks and 12 weeks.　Results　Six weeks and 12 weeks after operation, the concentration of magnesium ion in blood and urine was tested by first, two, four, eight and twelve weeks, respectively. X-ray and HE staining at the sixth and twelfth week showed that the femoral head of magnesium rod /BMSCs group had the best recovery, and at the twelfth week, the magnesium alloy rod was completely absorbed; the femoral head necrosis in the magnesium rod group and BMSCs group was slower, and some of the femoral head necrosis was improved.　Conclusion　Implantation of composite magnesium alloy-BMSCs-BMP-2 gene has effect on delaying femoral head necrosis and improving repair.

Key words: BMSCs, BMP-2, Magnesium alloy, Femoral head necrosis, Rabbits

ZHANG Yan-ru,ZHANG Hui, Kaka, ZHANG Ge-chen, SHANG Yan, SUN Hao, HUANG Wen-hua. BMSCs-BMP-2 composite with magnesium alloy rod in the repair of femoral head necrosis[J]. Chinese Journal Of Clinical Anatomy, 2016, 34(5): 528-533.

References

[1] 张雁儒，张辉，马辉, 等. BMP-2基因转染对兔骨髓间充质干细胞碱性磷酸酶活性和钙含量的影响[J]. 郑州大学学报医学版，2014，01.029(1): 1671-6825.
[2] Zhang YG, Yang Z, Zhang H, et al. Negative pressure technology enhances bone regeneration in rabbit skull defects[J]. BMC Musculoskelet Disord, 2013, 14(11):76-82.
[3] Song I, Kim BS, Kim CS，et al. Effects of BMP-2 and vitamin D3 on the osteogenic differentiation of adipose stem cells[J]. Biochem Biophys Res Co, 2011, 408(9):126-131.
[4] Xia Y. Bone tissue engineering using bone marrow stromal cells and an injectable sodium alginate/ gelatin scaffold [J]. J Biomed Mater Res A, 2012, 100(4): 1044-1050.
[5] Hattori T. Experimental investigation of osteogenesis and chondrogenesis by implant of BMP-fibrin glue mixture [J]. Nihon Seikeigeka Gakkai Zasshi, 1990, 64(9): 824-834.
[6] Balla VK, Bodhak S, Bose S, et al. Porous structures for bone implants: fabrication mechanical and in vitro biologicalerties[J]. Acta Biomater, 2010, 6(8):3349-3359.
[7] Jittapiromsak N, Sahawat D, Banlunara W, et al. Acemannan, an extracted product from Aloe vera, stimulates dental pulp cell proliferation, differentiation, mineralization, and dentin formation[J]. Tissue Eng Part A, 2010, 16(5):1997-2006.
[8] Jettanacheawchankit S, Sasithanasate S, Sangvanich P, et al. Acemannan stimulates gingival fibroblast proliferation; expressions of keratinocyte growth factor-1, vascular endothelial growth factor, and type I collagen; and wound healing[J]. J Pharmacol Sci, 2009, 109(4):525-531.
[9] 李子剑，张克，娄思权，等. 镁钙合金的细胞毒性研究[J].中国骨与关节损伤杂志，2007, 22(9): 740-742.
[10]Pengde K, Bin S, Jing Y, et al. Repairing defect and preventing collapse of canine femoral head using titanium implant enhanced by autogenous bone graft and rhBMP-2 [J]. Connective Tissue Res, 2007, 48(4): 171-179.
[11]Pei M, Shoukry M, Li J, et al. Modulation of in vitro microenvironment facilitates synovium-derived stem cell-based nucleus pulposus tissue regeneration [J]. Spine, 2012,37(18): 1538-1547.
[12]Takaoka K,Yoshioka T,Hosoya T,et al. The repair process in experimentally induced avascular necrosis of the femoral head in dogs[J]. Arch Orthop Trauma Surg, 1981, 99(2):109-115.

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BMSCs-BMP-2 composite with magnesium alloy rod in the repair of femoral head necrosis

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