大鼠急性甲醇中毒大脑皮质单核细胞趋化蛋白-1蛋白的表达

doi:10.13418/j.issn.1001-165x.2017.01.011

Abstract

Abstract:

Objective　This study was aimed to investigate the expression of monocyte chemoattractant protein-1 (MCP-1) proteinin rats after acute methanol poisoning.　Methods　Animal models of acute methanol poisoning in rats were duplicated using a plexiglas chamber exposed to a mixture of N2O/O2. Perfusion fixation, paraffin embedding, and sectioning were used, and positive cells expression was observed. Extract protein from brain tissue and Western blot were use to detected the expression level of MCP-1 protein.　Results　Results of Western blot: The expression level of MCP-1 were significantly enhanced with over time following acute poisoning, reaching the maximum at 24h; Expression in high-dose group was higher than low-dose group at 12h and 24h (P<0.01). There were no significant difference between the low-dose group and high-dose group at 3d (P>0.05). Results of immunohistochemistry experiment: MCP-1 positive cells reaction could be found in piriform cortex in the high-dose group, whereas the saline group showed no brown-yellow granules scattered. The expression level of MCP-1 were significantly increasedat 24 h (P<0.01), reached the maximum at 3d (P<0.01)，and then decreased at 1week, but still had significant difference compared with the saline control group (P<0.01).　Conclusion　The expression level of MCP-1 proteinare significantly enhanced and last a period of time following acute methanol poisoning; the degree of poisoning and the dose administered is apparently related, and MCP-1 might participate in brain pathological process.

Key words: Monocyte chemoattractant protein-1, Methanol poisoning, Rats

LI Fan，LI Hong-e，HAN Hong，CAO Xiao-qing，ZHAO Min，LI Juan-juan2，YUAN Yun，WU Chun-yun. Expression of monocyte chemoattractant protein-1 protein in cerebral cortex of rats after acute methanol poisoning[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(1): 52-56.

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Expression of monocyte chemoattractant protein-1 protein in cerebral cortex of rats after acute methanol poisoning

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