神经生长因子对大鼠跨区穿支皮瓣成活的影响

doi:10.13418/j.issn.1001-165x.2017.02.009

Abstract

Abstract:

Objective　To evaluate the nerve growth factor (NGF) effect on survival of dorsal cross-boundary perforator flap of SD rats.　Methods　60 adult SD rats were divided into the experimental group and the control group equally，in the dorsum of which a cross-boundary perforator flap with an area of about 3 cm×10 cm was harvested. The experimental group was injected subcutaneously with NGF solution (10 nmol·ml-1·kg-1); the control group was injected with 0.1 mol/L PBS solution (1ml/kg). 3 and 7 days after surgery, VEGF and CD34 of the local tissues of flap were tested via Blot Western；Angiography was performed by the micro-CT to detect Three-dimensional morphological alteration of microvasculature using factorial design analysis of vascular volume and total length. At day 7, survival area of the flap was measured before the observation of expression of KDR and TrkA in vasculature by immunohistochemical stain. Results Day 3, there was no statistically significant differences in the expression of VEGF between two groups (P=0.088); the expression of CD34 in the experimental group was higher than that in the control group (P=0.004); Day 7, there were higher expression of VEGF and CD34 in the experimental group (P<0.05)；Morphological analysis showed that there is an interaction between NGF and time in the increase of vascular volume (F=33.304, P<0.05) and the total length of vessels (F=8.493, P=0.01); Compared with the control group, the flap survival area of the experimental group was significantly higher (P<0.05). The expression of KDR in experimental group was markedly superior to the control group. Conclusion NGF can promote the survival of thecross-boundary perforator flap in SD rat.

Key words: Nerve growth factor, Cross-boundary flap, Perforator flap, Micro-computed tomography, Morphologic analysis

YANG Xi，Li Guo-dong，WANG Teng，HE Xiao-qing，XU Yong-qing. Effect of nerve growth factor on survival of cross-boundary perforator flap in rats[J]. Chinese Journal Of Clinical Anatomy, doi: 10.13418/j.issn.1001-165x.2017.02.009.

References

[1] Pohlenz P, Klatt J, Schon G, et al. Microvascular free flaps in head and neck surgery: complications and outcome of 1000 flaps[J]. Int J Oral Maxillofac Surg, 2012, 41(6):739-743.
[2] 庄跃宏，梁成，温福利，等. 延迟术通过加强血管扩张的方式促进跨区皮瓣存活[J]. 中国临床解剖学杂志, 2015，33(1):44-50.
[3] 谢红炬，林彪斌，李明，等. 碱性成纤维细胞生长因子共聚物缓释微球与兔跨区轴型皮瓣的成活[J]. 中国组织工程研究, 2012(47):8813-8816.
[4] Temiz G, Yesiloglu N, Sirinoglu H, et al. Increasing the survival of transverse rectus abdominis musculocutaneous flaps with a Botulinum toxin-A injection: A comparison of surgical and chemical flap delay methods[J]. J Plast Reconstr Aesthet Surg, 2016, 69(7):944-951.[5] Karatzas A, Katsanos K, Lilis I, et al. NGF promotes hemodynamic recovery in a rabbit hindlimb ischemic model through trkA- and VEGFR2-dependent pathways[J]. J Cardiovasc Pharmacol, 2013, 62(3):270-277.
[6] Zeng W, Yuan W, Li L, et al. The promotion of endothelial progenitor cells recruitment by nerve growth factors in tissue-engineered blood vessels[J]. Biomaterials, 2010, 31(7):1636-1645.
[7] Taylor GI, Corlett RJ, Dhar SC, et al. The anatomical (angiosome) and clinical territories of cutaneous perforating arteries: development of the concept and designing safe flaps[J]. Plast Reconstr Surg, 2011,127(4):1447-1459.
[8] 陈世新，吴东方，丁茂超，等. 穿支体区血管及其相互间吻合的3D可视化研究[J]. 中国临床解剖学杂志, 2011，29(3):237-242.
[9] 薛兰，吴志海，汤莹莹，等. 激光多普勒对大鼠背部3种皮瓣模型的血流动力学特点研究[J]. 中国临床解剖学杂志, 2016，34(1):6-11.
[10] Schambach SJ, Bag S, Groden C, et al. Vascular imaging in small rodents using micro-CT[J]. Methods, 2010, 50(1):26-35.
[11] Hu JZ, Wu TD, Zhang T, et al. Three-dimensional alteration of microvasculature in a rat model of traumatic spinal cord injury[J]. J Neurosci Methods, 2012, 204(1):150-158.
[12]胡斯旺，毛海蛟，庄跃宏，等. 再探大鼠背部穿支体区间choke血管变化规律[J]. 中国临床解剖学杂志, 2014，33(6):690-693.
[13]Diao YP, Cui FK, Yan S, et al. Nerve growth factor promotes angiogenesis and skeletal muscle fiber remodeling in a murine model of hindlimb ischemia[J]. Chin Med J (Engl), 2016, 129(3):313-319.
[14]Chen WH, Mao CQ, Zhuo L L, et al. Beta-nerve growth factor promotes neurogenesis and angiogenesis during the repair of bone defects[J]. Neural Regen Res, 2015, 10(7):1159-1165.
[15]习珊珊，丁茂超，杨大平，等. SDF-1及其受体CXCR4在皮瓣外科中的研究进展[J]. 中国临床解剖学杂志, 2016, 34(2):232-234.
[16] 吴建龙，巨积辉，周广良，等. SB202190对皮瓣缺血再灌注损伤时TNF-α和IL-10影响的实验研究[J]. 中国临床解剖学杂志, 2014, 32(6):698-703.
[17] 郭伟新. NGF对脑梗死患者内皮祖细胞的影响及其机制研究[D]. 中南大学, 2008.

Effect of nerve growth factor on survival of cross-boundary perforator flap in rats

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	Yue Qin, Chen Rongli, Xiong Jingxi, Wang Tingyi, Cai Zhiheng, Yi Qiushi, Zeng Xinyi. Effect of Bletilla striata polysaccharides on the choke-area of cross-boundary flap in rats [J]. Chinese Journal of Clinical Anatomy, 2024, 42(1): 59-64.
[2]	Song Hehua#, Zhang Qi#, Wu Bihua, Chen Wei, Zhang Tianhua, Nie Kaiyu, Chang Shusen, Deng Chengliang, Li Hai, Zhou Jian, Li Shujun, Wei Zairong. Study on the relationship between the grade IV perforator’s vessels and the survival of reticular blood-supplying flap [J]. Chinese Journal of Clinical Anatomy, 2023, 41(3): 288-295.
[3]	He Xiaoqing, Yang Xi, Shi Yan, Xu Yuexian, Fang Xiang, Fan Xinyu, Wu Yaling, Xu Yongqing. Anatomical study and clinical significance of superficial lateral sural artery perforator flap [J]. Chinese Journal of Clinical Anatomy, 2023, 41(2): 132-136.
[4]	Qiu Yujun, Fu Yu, Su Hang, Yin Siyuan, Wang Yueqing, Tang Maolin, Xi Shanshan. Influence of pedicled artery position on the survival of multi-territory perforator flap [J]. Chinese Journal of Clinical Anatomy, 2022, 40(6): 660-664.
[5]	Wang Yining, Fan Xinyu, Bi Xin, Peng Yufeng, Bi Zhenyu. Clinical study on repairing large tissue defect of lower limbs with the perforator flaps in anterolateral wall abdominal [J]. Chinese Journal of Clinical Anatomy, 2022, 40(6): 725-729.
[6]	Sun Yu, Yang Lixue, Wang Guanlin, Zhu Wei, Qiu Yongfeng , Liang Haohao. The clinical application of superficial circumflex iliac artery perforator flaps in repairing soft tissue defects of extremities under the concept of "swimming trunks donor area" [J]. Chinese Journal of Clinical Anatomy, 2022, 40(5): 587-591.
[7]	Deng Changhua, Kong Fanfu, Wu Zhen, Nie Meng, Liu Chao. Clinical application of the perforator flap based on the first perforating branch of profunda femoral artery in reconstruction of patients with IV degree pressure ulcers in the region of buttock and thigh [J]. Chinese Journal of Clinical Anatomy, 2021, 39(3): 351-354.
[8]	Kang Yongqiang, Rui Yongjun, Wu Yongwei, Ma Yunhong, Liu Jun, Gu Jun, Xu Peng, Lin Fang. Repair soft tissue defects of distal termination of leg and around the foot and ankle with perforators positioning technique assisited by color dopplerultrasound [J]. Chinese Journal of Clinical Anatomy, 2021, 39(2): 202-206.
[9]	ZHANG Guo-lei, LI Wen-qing, ZHU Xiao-di, WANG Wen-sheng, TAN Rong-zhi. Application of handheld Doppler ultrasound in repairing foot defect with anterolateral thigh flap [J]. Chinese Journal of Clinical Anatomy, 2020, 38(5): 597-599.
[10]	WANG Tian-quan, HU De-qing, LIN Hai-qing, WEN Fu-li, XUE Lai-en. Anatomical basis of the dorsal perforating extended flap based on the palmar artery at the site between the thumb and the index finger [J]. Chinese Journal of Clinical Anatomy, 2020, 38(2): 127-130.
[11]	HU De-qing, ZHOU Xiao, CHEN Peng, ZHANG Wen-ming. Anatomical study of the V-Y advancement flap based on the cubital fossa perforators [J]. Chinese Journal of Clinical Anatomy, 2020, 38(2): 131-134.
[12]	HU De-qing, ZHOU Xiao, CHEN Peng, ZHANG Wen-ming. Anatomical study of the semimembranosus popliteal perforator flap [J]. Chinese Journal of Clinical Anatomy, 2020, 38(2): 135-137.
[13]	QI Rui-lin, LIN Hai-qing, XUE Lai-en, XIONG Zhi-gang. Anatomical study of the axillary hair-bearing perforator flap [J]. Chinese Journal of Clinical Anatomy, 2020, 38(2): 138-141.
[14]	LIN Hai-qing, QI Rui-lin, XUE Lai-en, WEN Fu-li, XIONG Zhi-gang. Clinical anatomy of the pubic hair-bearing perforator flap [J]. Chinese Journal of Clinical Anatomy, 2020, 38(2): 142-145.
[15]	DENG Chang-hua, KONG Fan-fu, WU Zhen, NIE Meng, QI Zhan-jun. Repair of skin and soft tissue defects around ankle with modified mid-low propeller perforator flap of peroneal artery [J]. Chinese Journal of Clinical Anatomy, 2019, 37(4): 454-456.