髓样细胞特异性SETD4基因敲除小鼠的构建与鉴定

doi:10.13418/j.issn.1001-165x.2017.02.012

Abstract

Abstract:

Objective　To elucidate the function of SETD4, we established myeloid cell-specific SETD4 knockout mice. 　Methods　Setd4flox/+ mice were inbred to obtain Setd4flox/flox mice, which then bred with FLP mice to obtain the Setd4fl/+/flp mice. After crossing with C57BL/6 mice, Setd4fl/+ mice were obtained, while Setd4fl/+/Lyz2-Cre were obtained after crossing with Lyz2-Cre mice. And then Setd4-/-/Lyz2-Cre mice were generated by mating Setd4fl/+/Lyz2-Cre with Setd4fl/+ mice. PCR was used to identify the genotype of offspring. The mRNA level of SETD4 in peritoneal macrophages and liver were detected by quantitative real time PCR to confirm the knockout efficiency.　Result　The mRNA expression of SETD4 in peritoneal macrophages of myeloid cell-specific SETD4 knockout mice was significantly lower than the wildtype mice, while no significant difference could be detected in the liver.　Conclusion　Based on FLP/FRT、Cre/Loxp recombination system, myeloid cell-specific SETD4 knockout mice were successfully established for further research.

Key words: FLP/FRT, Cre/loxp, SETD4, Gene knockout

HUANG Meng-yi, ZHONG Yu-yun, HUANG Sui, Sun Jiang, Li Yue, WANG Juan, JIANG Yong, LIU Jing-hua. Establishment and identification of myeloid cell-specific SETD4 knockout mice[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(2): 177-182.

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Establishment and identification of myeloid cell-specific SETD4 knockout mice

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