黄连素对缺氧复氧诱导大鼠心肌细胞内Nrf2/Keap1的影响

doi:10.13418/j.issn.1001-165x.2017.03.010

Chinese Journal Of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (3): 282-285.doi: 10.13418/j.issn.1001-165x.2017.03.010

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Effects of berberine on the expression of Nrf2/Keap1 in hypoxia reoxygenation induced myocardial cells

WANG Song-hai¹, CHE Yi-hong², CHEN Jie¹

1. Shaanxi Province Hospital of TCM， Shaanxi, Xi'an 710002，China； 2. Hanzhong Central Hospital, Hanzhong, Xi'an 723000, China

Received:2016-10-30 Online:2017-05-25 Published:2017-06-23

Abstract

Abstract:

Objective　To examine the effect of berberine on hypoxia reoxygenation induced apoptosis in myocardial cells and to explore the potential mechanisms involved.　Methods　H9C2 cardiac muscle cells were divided into: normal control group (NC group), simple berberine group (BR group), hypoxia reoxygenation group (HR group) and drug treatment group (divided into LBR group: 1.5×10-6 mol/L, HBR group 1.5×10-4 mol/L). Myocardial cell vitality was detected by MTT, nucleus morphological changes were examined using Hoechst 33258 dye and Nrf2 and Keap1 protein expressions were detected by Western blot.　Results　Compared with that in NC group, simple berberine exerted no effect on myocardial cells in BR group. Compared with those in HR group, the cell vitality in treatment groups increased((65.2±1.6)%; (82.3±1.4)%; P<0.01)，and apoptosis decreased significantly(P<0.05). Western blot results showed that berberine increased the expression of the antioxidant-related protein Nrf2, and reduced the level of Keap1. Conclusion　Berberine has significant protective effect on hypoxia reoxygenation induced apoptosis in myocardial cells possibly by enhancing the release of Nrf2 and decreasing the expression of Keap1.

Key words: Berberine, 　Hypoxia reoxygenation, 　Cardiac muscle cells

WANG Song-hai, CHE Yi-hong, CHEN Jie. Effects of berberine on the expression of Nrf2/Keap1 in hypoxia reoxygenation induced myocardial cells[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(3): 282-285.

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Effects of berberine on the expression of Nrf2/Keap1 in hypoxia reoxygenation induced myocardial cells

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