Detection of bone metastasis by small animal micro-PET/CT scan in nude mouse prostate cancer bone metastasis animal models
ZHONG Zhi-hong, ZHOU Wei, YU Hao-tao, YAN Wei-ming, SHEN Hua-wei, ZHANG Pin
Chinese Journal of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (3) : 296-300.
Detection of bone metastasis by small animal micro-PET/CT scan in nude mouse prostate cancer bone metastasis animal models
Objective To investigate the viability of small animal micro-PET/CT scan for detection of prostate cancer bone metastasis in nude mouse prostate cancer bone metastasis models. Methods 23 nude mice were divided into control group (2 mice),orthotopic injection group(7 mice),intracardiac injection group(7 mice),intratibial injection group(7 mice), randomly. 40 days after the tumor cells injection, the animal were taken for PET/CT scanning (orthotopic injection group &intratibial injection group) and high-resolution micro-CT scanning(intratibial injection group). After the examination, the animals were sacrificed and dissected to identify metastasis tumor tissue. Suspicious bone metastasis was fixed in 10% formalin for histological examination. Results In the orthotopic injection group, no obvious bone destruction was detected (0/7). In the intracardiac injection model, one upper tibia bone destruction was detected by PET/CT scanning, which was confirmed by the high-resolution micro-CT scanning and HE stain; In the intratibial injection model, all animals formed various degrees of bone destruction(7/7) and the high-resolution micro-CT scanning showed various degrees of bone destruction clearly. Conclusion PET/CT scanning can identify the location of bone metastasis and the degrees of bone destruction clearly, which can serve as a promising method for cancer bone metastasis research.
Prostate cancer bone metastasis / Animal model / Small animal micro-PET/CT scan
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