LSCI检测不同缺血期对大鼠缺血再灌注过程脑血流的影响

doi:10.13418/j.issn.1001-165x.2017.04.011

Chinese Journal Of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (4): 409-412.doi: 10.13418/j.issn.1001-165x.2017.04.011

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Effects of different ischemic duration on post-ischemic and reperfusion rat cerebral blood flow detected by LSCI

TIAN Zhi-hui¹, LIAO Wei-guo², YU Jian-ye², WANG Li-sheng²

1.Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; 2. College of Chinese Traditional Medicine, Guangzhou University of Chinese Medicine, Guangzhou 500006, China

Received:2017-03-23 Online:2017-07-25 Published:2017-08-30

Abstract

Abstract:

Objective　To investigate the effects of different ischemic duration on post-ischemic and reperfusion rat cerebral blood flow.　Methods　Laser speckle imaging system (LSCI) was used to detect the effects of different ischemic duration on post-ischemia and reperfusion rat cerebral blood flow. At the same time, the dynamic changes of capillary flow and diameter, no reflow phenomenon and hypoperfusion were analyzed.　Results　During 2h middle cerebral artery occlusion (MCAO) ischemia, perfusion of cerebral blood flow decreased to (35±10)% of the baseline, reflecting a state of hypoperfusion. With the prolongation of ischemic time, no reflow phenomenon was aggravated. Hypoperfusion in 2 h group was approximately 10% less than that in 30 min group during the period of ischemia and reperfusion.　Conclusion　The long ischemic duration on post-ischemic and reperfusion may be one of the main reasons for the increase of the degree of brain injury.The established LSCI method can be applied to real-time monitoring of MCAO rat cerebral blood flow, which is easy to operate, sensitive, stable and reliable.

Key words: Cerebral ischemia-reperfusion, 　Laser speckle imaging, 　Cerebral blood flow

TIAN Zhi-hui, LIAO Wei-guo, YU Jian-ye, WANG Li-sheng. Effects of different ischemic duration on post-ischemic and reperfusion rat cerebral blood flow detected by LSCI[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(4): 409-412.

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Effects of different ischemic duration on post-ischemic and reperfusion rat cerebral blood flow detected by LSCI

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