枸杞多糖通过泛素蛋白酶体途径降解突变亨廷顿蛋白

doi:10.13418/j.issn.1001-165x.2017.06.010

Chinese Journal Of Clinical Anatomy ›› 2017, Vol. 35 ›› Issue (6): 641-644.doi: 10.13418/j.issn.1001-165x.2017.06.010

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Lycium barbarum polysaccharide degrades the mutant huntingtin by ubiquitin-proteasome pathway

FANG Fang ^{1, 2}, CHEN Tian¹, PENG Yun-tao ¹, LI He²

1. Department of Human Anatomy, Guilin Medical University, Guilin 541004, China; 2. Division of Histology and Embryology, Department of Anatomy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

Received:2017-07-26 Online:2017-11-25 Published:2017-12-30

Abstract

Abstract:

Objective　To detect the degradation pathway of the mutant huntingtin (mHtt) after treating Lycium barbarum polysaccharide (LBP).　Methods　HEK293 cells that stably expressed mHtt containing 160 glutamine repeats was treated with LBP of various doses, CCK8 assay was used to detect the cell viability, and caspase-3 activity assay kit was used to detect the caspase-3 activity; the mHtt in HEK293-160Q cells was observed under fluorescence microscope after treatment with LBP and the images were analyzed by Image Pro Plus 6.0, at the same time, to detect its mRNA; LBP, MG132 and chloroquine were used to treat HEK293-160Q cells separately, and the changes of the mHtt were determined by Western Blot. Results　LBP could increase the viability and decrease the caspase-3 activity in HEK293-160Q cells; LBP could reduce the mHtt and did not change its mRNA levels; after treating HEK293-160Q cells with the different medicines , the Western Blot results showed that compared to cells receiving only LBP treatment, the degeneration of mHtt was significantly reduced after treating LBP and MG132. No influence on mHtt after treating LBP and chloroquine was observed.　Conclusions　LBP can degrade the mHtt by ubiquitin-proteasome pathway, increase the cell viability and decrease the apoptosis by alleviating the cytotoxicity of mHtt.

Key words: Mutant huntingtin;　Lycium barbarum polysaccharide;　Ubiquitin-proteasome pathway; , Autophagy-lysosome pathway

FANG Fang, CHEN Tian, PENG Yun-tao, LI He. Lycium barbarum polysaccharide degrades the mutant huntingtin by ubiquitin-proteasome pathway[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(6): 641-644.

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Lycium barbarum polysaccharide degrades the mutant huntingtin by ubiquitin-proteasome pathway

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