促红细胞生成素导致肥胖小鼠棕色脂肪PPAR γ、UCP1和β3-AR的表达变化

doi:10.13418/j.issn.1001-165x.2017.06.011

Abstract

Abstract:

Objective　Erythropoietin (EPO) has been reported to have a beneficial effect on obesity. The aim of the study is to investigate the changes of body weight and fat content of EPO in high fat diet (HFD) mice, the expression of PPAR γ, UCP1, β3-AR mRNA in white adipose tissue (WAT) and brown adipose tissue (BAT). Methods 20 HFD-fed C57BL/6J male mice were randomly divided into control group (HFD-Con) and EPO group (HFD-EPO). The expression of PPAR γ, UCP1 and β3-AR mRNA in subcutaneous fat, epididymal fat and brown fat were detected by real-time quantitative RT-PCR. Results Compared with the control group, the body weight of the EPO mice was (24.53±2.03) g and the control group was (31.78±1.68) g. The weight of subcutaneous fat and epididymal fat in HFD-EPO group were significantly decreased. HE staining showed that the size of adipocytes of HFD-EPO group was decreased compared with HFD-Con group. Quantitative real-time PCR results showed that the expression of PPAR γ, UCP1 and β3-AR mRNA in subcutaneous adipose tissue and epididymal adipose tissue did not change significantly, while the expression of PPAR γ, UCP1 and β3-AR in brown fat were significantly increased. Conclusion　The expression of PPAR γ, UCP1 and β3-AR in brown fat may be related to the weight loss caused by EPO injection after high fat diet.

Key words: High fat diet, 　Erythropoietin, 　Peroxisome proliferator-activated receptors &gamma, 　Uncoupling protein 1, 　&beta, 3-adrenergic receptor

MIAO Ying-ying, LU Ming, ZHAO Jun-qiang. Changes of PPAR γ, UCP1 and β3-AR mRNA expression of HFD-induced mice with EPO injection[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(6): 645-649.

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Changes of PPAR γ, UCP1 and β3-AR mRNA expression of HFD-induced mice with EPO injection

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