少阳主骨方对食蟹猴自然退变膝关节骨性关节炎模型p16/Rb信号通路的影响

doi:10.13418/j.issn.1001-165x.2017.06.013

Abstract

Abstract:

Objective　 The aim of this study was to investigate the mechanism of ShaoYangZhuGuFang(SYZGF) in delaying the cell senescence and degeneration of articular chondrocytes according to the theory of "ShaoYangZhuGu". Method 13 cynomolgus monkeys with degenerative knee joints were selected by X-ray. One of the monkeys was selected randomly for pathologic observation, and the other cynomolgus monkeys were randomly divided into SYZGF group, Ammonia Moxime group and Saline group. There were four cynomolgus monkeys in each group. All cynomolgus monkeys were executed after 8 consecutive weeks of intragastric administration. Pathological observation of articular cartilage was made and the expression of p16, Rb gene and protein in articular cartilage was detected by RT-qPCR and Western-blot technique.　Result　Articular cartilage pathological changes of elderly cynomolgus monkey KOA models was consistent with the characteristics of KOA pathological changes. Mankin scores of cynomolgus monkeys in three groups were as follows: 7.5 ± 0.53 in SYZGF group, 7.75 ± 0.71 in Ammonia Moxime group, 8.25 ± 0.46 in Saline group, and there was significant difference between SYZGF group and Saline group (P<0.05). The expression of p16 was lowest in SYZGF group, which in middle in Ammonia Moxime group, and highest in Saline group. There was significant difference in the three groups (P<0.05). The expression of Rb was highest in SYZGF group, middle in Ammonia Moxime group, and lowest in Saline group. There was significant difference in the three groups (P<0.05).　Conclusion　SYZGF could delay chondrocyte senescence by p16/Rb pathway and delay articular cartilage degeneration.

Key words: ShaoYangZhuGu, 　Cynomolgus monkeys knee osteoarthritis model, 　Cell senescence, p16/Rb signaling pathway

ZHOU Xin, ZHANG Lei, FU Shi-jie, LIU Gang, GUO Xiao-guang, WANG Guo-you. Effects of ShaoYangZhuGuFang on p16/Rb signaling pathway of naturally degenerative cynomolgus monkeys knee osteoarthritis model[J]. Chinese Journal Of Clinical Anatomy, 2017, 35(6): 655-660.

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Effects of ShaoYangZhuGuFang on p16/Rb signaling pathway of naturally degenerative cynomolgus monkeys knee osteoarthritis model

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