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Chinese Journal of Clinical Anatomy
(Founded in July 1983 Bimonthly)
Responsible Institution: China Association for Science and Technology
Sponsor: Chinese Society of Anatomical Sciences
Editing and Publishing: Editorial Board of Chinese Journal of Clinical Anatomy
Advisor: ZHONG Shi-zhen
Editor-in-chief: OUYANG Jun
Former Editor-in-chief: XU Da-chuan
Overseas Distributor: China International Book Trading Corporation (P.O. Box 399, Beijing, China, Code No. BM5961)
Address: 1023 Shatainan Road, Guangzhou, 510515, China
Tel: (020)61648203
E-mail: journal@chjcana.com
CN: 44-1153/R
ISSN: 1001-165X

Table of Content

    25 May 2014 Volume 32 Issue 3
      
    3D printing technology assists the development of clinical medicine
    LI Jian-Die
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  241-242.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 443 )  
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    The preliminary application of digital technology in the treatment of calcaneusmalunion
    MEI Gang, TUN Wei-Dong, OU Yang-Jun, JIN Dan, FU Su, JU Zhen-Lv
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  243-247.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 459 )  
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    Objective To explore the virtual reconstruction technique of calcaneal malunion digitization and the feasibility of the method applied in the design of orthopedic surgery.     Methods CT scan images of ipsilateral, contralateral foot and ankle of calcaneal malunion cases were imported to Mimics14.0 for reconstruction of the 3D model. on the basis of the reconstruction and measurement of 3D model, virtual osteotomy and digital fixation surgery were designed. After the digital surgery, anatomical parameters were compared to ipsilateral calcaneus. if the results are acceptable, then surgery was operated. Results    3D visualization model of the calcaneal malunion was constructed and anatomical parameters were measured and then 3D physical model was also printed by rapid prototyping machine. Personal computer could be used to perform virtual osteotomy and internal fixation. Conclusions   Digital virtualized reconstructive technique could be used to provide precisely the anatomical and radiological data for judgment of the calcaneal malunion and decision of clinical diagnosis and treatment methods, and it also holds great promise for preoperative design of calcaneal malunion surgery.

    The application of 3D printing technique in the research of acetabular fractures
    TUN Zhang-Lin, LIN Hai-Bin, ZHANG Guo-Dong, LIU Jian-Jun, CHEN Xuan-Huang, HUANG Hua-Jun, HUANG Wen-Hua
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  248-251.  doi:10.13418/j.issn.1001-165x.201
    Abstract ( 407 )  
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    Objective    To evaluate the digital design of acetabular fractures and the feasibility of the implementation, then using the 3D printing technique to verify the results.    Methods    Fifty-two patients with acetabular fractures were enrolled in this study. Data of lamellar CT scanning were imported into Mimics14.0 software for 3D anatomical modeling and virtual fracture reduction. Then the default embedded steel plate location was optimized , the screw path evaluated,and the screw length measured. 3D printing technique was used to print the bone. Plates were bended based on the printing bones. Internal fixation implants were simulated based on all the printed bones and bended plates. Finally, the real operation was compared with virtual preoperative design in terms of the fixation position of plates, the direction and the length of screws. Results    In the study, 61 plates and 424 screws were planted. The position of plates and the direction of screws were in high accordance with the digital design. The length of screws are compared with Paired-samples t Test and P=0.325.   Conclusion    Firstly, the combination of the digital design and 3D printing technique have a good application in the bending of the plates and surgery simulation before operation. The real operation is quite consistent with the simulated results. Secondly, plate bending before the real operation has a certain value in the guidance of fracture reduction.

    Experimental study on accurate placement of the lumbar pedicle screws assisted by 3D printing navigation module
    YAN Bin, ZHANG Guo-Dong, TUN Zhang-Lin, HU Jing, YANG Xiang, HUANG Wen-Hua
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  252-255.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 545 )  
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    Objective To study individualized navigation module that can realize the accurate placement of the minimally invasive lumbar pedicle screws based on digital design and 3D printing technology. Methods 37 cases of the normal human lumbar (L1~L5) 3D model were reconstructed in our study. Navigation bars were designed through the pedicle center. Based on the navigation bars, navigation modules were produced by Boolean Operation. The entities of navigation modules and lumbar spine specimens were built by using 3D printing technology. Then, pedicle screws assisted by navigation modules were implanted. Finally, the anchor point,orientation and position of the screws were observed. Results Navigation modules were used in 228 cases of pedicle screws implantation. The anchor point and orientation of the screws were all consistent with virtual schemes. The position of pedicle screws were good and none of the screws worn out the pedicle cortex. Conclusion By means of digital design and 3D printing navigation module, accurate placement of individualized minimally invasive lumbar pedicle screws can be realized.

    Digital production of a novel liver casts using rapid prototyping and mold technologies
    KONG Xiang-Xue, NIE Lan-Yang, XIAO Ju-Jiao, LIU Fu-Yu, LI Jian-Die
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  256-258.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 582 )  
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    Objectives To explore a novel hepatic duct cast with transparent liver parenchyma and internal ducts.    Methods   A fresh human liver was harvested and the hepatic and portal veins were perfused. And the sectional images were obtained by a 64-slice spiral CT. The three-dimensional (3D) models of the portal vein, hepatic vein and liver parenchyma were reconstructed using Mimics software. The shell molds of the liver parenchyma and its connection with ducts were designed in software Magic 4.0. The 3D models were then uploaded into a rapid prototype 3D printer to physically duplicate the models. After assembling the cast and the shell structures, the mold was manufactured. Melted transparent jelly wax was then poured into the mold. After wax solidification, the shell molds were removed. And the novel RP(rapid prototype) hepatic cast was produced.    Result    The novel RP cast of human liver with transparent parenchyma and internal ducts was successfully produced. It had a fine morphology and a good hand feeling with the transparent jelly wax. And the duct structures of the portal vein and hepatic vein were clearly displayed in different colors with their intimate relationship demonstrated. The smallest branch of the RP cast of hepatic ducts was about 1.2 mm in diameter.     Conclusion    The RP cast of human liver can demonstrate the details of the liver structure and is convenient at reservation It can expand the application of the duct cast in medical education, research and clinical trials.

    Anatomical basis for design of the chimeric fibular composite flap based on the peroneal artery
    XIE Zhi-Beng, PENG Ti-Hong, ZHENG He-Beng, ZHANG Yi-Xin, LIANG Cheng, HAO Pan-De, ZHANG Fa-Hui
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  259-263.  doi:10.13418/j.issn.1001-165x.201
    Abstract ( 482 )  
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    Objective To provide anatomical basis for design of the chimeric fibular composite flap based on the peroneal artery. Methods 30 cadaveric lower extremities were used for this study. The fibular head and the lateral malleolus were used as the landmarks and the calves were divided into the upper, middle and lower segments, respectively. Dissection of the calves was carried out and the following contents were investigated: ①The origin, course and branches of the peroneal arteries; ②The number of the musculocutaneous or septocutaneous perforators of the three segments and the periosteal branches from the peroneal arteries.   Results   The peroneal  artery originated from the tibial artery and travelled immediately posteriorly to the fibular bone after it coursed to the middle segment of the fibular. It continued as the lateral calcaneal artery, giving off the branches to nourish the adjacent bones, muscles, and the skin on the lateral aspect of the lower leg. Among the branches given off, 2 to 8 branches were to the periosteum of the middle and lower 1/3 of the fibular bone, averaging 0.5 to 1.1 in diameter, which were termed fibular periosteal arteries; 1 to 3 branches were to the middle and lower 1/3 of the tibial periosteum, averaging 0.6 to 0.8 in diameter, which were termed tibial periostal arteries; 4 to 8 branches were the musculocutaneous or septocutnaoues perforators supplying the skin on the lateral aspect of the middle and lower segments of the lower leg. After piercing the deep fascia, the septocutaneous perforators anastomosed with the perforators from the anterior and posterior tibial arteries. Conclusions  It is anatomically feasible to harvest the chimeric fibular composite flap based on the peroneal artery, depending on the practical clinical needs;one type or multiple types of chimeric flaps can be harvested to reconstruct combined defects of bone and soft tissue.

    Applied anatomic study of video-assisted thoracoscopic ligation of thoracic duct
    ZHANG Xiao-Wen, YANG Jie, LIU Wen-Guo, HUANG Feng, WANG Ti-Jian, ZHANG Bin, TUN Jiao-Lian, GU Wei-Quan, XIE Dun, WANG Fei
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  264-268.  doi:10.13418/j.issn.1001-165x.201
    Abstract ( 517 )  
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    Objective To provide morphological basis for ligation of thoracic duct assisted by thoracoscopy. Methods An anatomical study was performed on ten normal adult cadavers to observe the original part of thoracic duct, cisterna chili, intestinal trunk, left and right lumbar trunks, the anatomical characteristics and distribution of the thoracic duct in the chest; and the distance from thoracic duct to the azygos vein, the hemiazygos vein and the accessory hemiazygos vein in the T4~12 segments was measured. Results  ①The thoracic duct mostly ascended from the cisterna chili medial to  the right diaphragmatic crus at the level of T12~L2 (one exception at T10). The distance from the origin of thoracic duct to the origin right diaphragmatic crus was (74.63±38.30) mm. There were all kinds of links among the intestinal trunk, left and right lumbar trunks, thoracic duct, the azygos vein and the accessory azygos vein. ② The minimum distance from the thoracic duct to the azygos vein and the maximum distance from the thoracic duct to the hemiazygos vein and the accessory hemiazygos vein was at the level of T8, and the distance grew and shrunk with the rib level rising and lowering, respectively.    Conclusions    In the course of T5~10 the thoracic duct has a fixed and single trunk with fewer variations. It is easy to dissect thoracic duct below the azygos vein, above descending aorta in front of vertebral column through trans-right-thoracic approach during video-assisted thoracoscopic surgery (VATS), while through trans-left-thoracic approach it should be above the azygos vein and below descending aorta.

    A case of suprascapular artery with initiative positional variation
    ZHANG Qi, LI Shen-Xiao, FU Feng, YANG Xin, XIAO Pei, DAI Xiao-Yan, FU Sheng-Qi
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  268.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 248 )  
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    The applied anatomy of lymphatics around the great saphenous vein
    BO Wei-Ren, WANG De-An, CHEN Yuan, CENG Fan-Jiang
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  269-274.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 605 )  
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    Objective To determine the lymphatic anatomy around the great saphenous vein (GSV) for the treatment of secondary lymphoedema in the lower extremity. Methods A total of six lower extremities from three unembalmed human cadavers were studied. Under a surgical microscope, 6% hydrogen peroxide was used to detect the lymphatic vessels commencing on the skin between the medial malleolus and the Achilles tendon. A 30-gauge needle was inserted into the vessels and injected with a radio-opaque barium sulphate or lead oxide mixture. The vessels were traced, photographed and radiographed to demonstrate the lymphatic pathways around GSV in the lower extremity. Results Numerous lymphatic vessels were identified in the subcutaneous tissue around GSV travelling in the medial side of the lower extremity. They originated  underneath the dermis between the medial malleolus and the Achilles tendon, travelling towards the superficial inguinal lymph nodes. The diameters of the vessels varied from 0.2 to 1.8 mm. During their tortuous course some vessels branched, diverged and converged, and sometimes anastomosed with neighboring vessels or crossed them. Most vessels converged to form larger collectors and then diverged into small branches before entering the lymph nodes. The nutrient vessels of the lymphatic vessels were found in 1 case. Conclusion Actual and accurate lymphatic routes from the skin between the medial malleolus and the Achilles tendon to the inguinal lymph nodes have been described. This information upgrades our anatomical knowledge and the results will be of bene?t for the clinical management of lymphedema and the other lymph-related diseases of the lower extremity.

    Anatomy and clinical research of ilioinguinal approach through retroperitoneal space for treatment of massive hemorrhage in pelvic fracture
    LIU Zhen-Xin, SHI Ceng-Yuan, YIN Wei-Gang, MAO Hai-Jiao, ZUO Rong-Ti-?
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  275-279.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 515 )  
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    Objective To study clinical anatomy and application of ilioinguinal approach through retroperitoneal space in the management of massive hemorrhage from pelvic fractures.  Methods The retroperitoneal space was exposed by both left and right ilioinguinal approaches on 37cadaver specimens, and parameters as follows were measured: the route and relationship of vessels and ureters; the anterior translation of inferior mesenteric artery, ureters, and testicular or ovarian artery and vena. The ilioinguinal approach was clinically performed for 12 cases of massive hemorrhage from pelvic fractures, 10 males and 2 females.  Results    The abdominal aorta, inferior vena cava, and common iliac artery and vena, in the retroperitoneal space, were firmly attached to the pelvic wall, and separated from the peritoneum with extraperitoneal fat; inferior mesenteric artery, testicular or ovarian artery and vena and parietal peritoneum are connected with each other by dense connective tissue, and could be lifted with parietal peritoneum with ease; the upper end of ureter has connection with parietal peritoneum, with the lower end loosely connected to it. The inferior mesenteric artery could  be anteriorly translated by 19.3 mm on the plane of L4, while the testicular or ovarian artery and vena could be anteriorly translated by 26.4 mm(right) and 28.0 mm(left), and the ureters 37.0 mm(left) and 41.0 mm(right). The ilioinguinal approach was clinically performed for 12 cases of massive hemorrhage from pelvic fractures, through the procedure of which the abdominal aorta compression was conducted to temporarily control the massive hemorrhage, with the shock rescued and corrected, the damaged vessels ligated and repaired, and the fractures fixed. There were 11cases of success and 1 case of death; on the whole, no vascular, nerve and vascular intimal injuries occurred.    Conclusions    The ilioinguinal approach through retroperitoneal space can completely expose major vessels in pelvis;  temporary abdominal aorta compression is a rapid and an effective way to control massive hemorrhage and correct shock, increasing the success rate in treatment of massive hemorrhage from pelvic fractures. This operative approach proves effective in rescuing patients with pelvic fractures complicated with large blood vessel injury.

    Measurements anatomical paremeters related to the L5/S1 axial intervertebral fusion based on 3D reconstruction technology
    LI Yang, LIU Yan, SUN Chao, DING Zi-Hai, TUN Li-Jun
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  280-283.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 451 )  
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    Objective  To provide the anatomical basis for the surgery approach of the axial lumbosacral interbody fusion. Methods The data of spiral CT scan images were imported into the Mimics 10.01 software in Dicom format, and the sacral vertebrae 3D geometric mesh model was obtained after 3D treatment. Finally, the Geomagic studio 12 was used to simply optimize the 3D model after importing in the STL format, and measure the relevant data. Results In the  AxiaLIF surgery which use the coccygeal tip as the entrance point and place the axial screw on the axle of lumbosacral vertebral bodies, the distance between the coccygeal tip and the entrance of the nail was (101.7±9.5)mm and (100.2±9.0)mm in Chinese males and females, respectively(P>0.05). In the AxiaLIF surgery which use the coccygeal incisures as the entrance point and place the axial screw on the middle column of the lumbosacral vertebrae, the distance between the coccygeal incisures and the entrance of the nail was  (82.4±9.5)mm and (83.1±10.6)mm in Chinese males and females, respectively(P>0.05). In  thetwo different surgical approaches of the AxiaLIF, the vertical distance between needle and S3/4, are (24.4±5.3)mm and (14.5±2.8)mm respectively in men (P<0.05), and are (27.0±5.6)mm and (17.0±3.8)mm respectively in women (P<0.05). There are significant statistical difference between the data of male and female groups. Conclusions There is high risk on the AxiaLIF which use the coccygeal tip as the entrance point. The new AxiaLIF surgery, which use the coccygeal incisures as entrance point  and place the axial screw on the middle column of lumbosacral vertebra, is in the safety range of the presacral space, and needs further verified by biomechanical study.

    The three-dimensional measurements and clinical significance of the morphology of posterior proximal tibia
    XIONG Ran, ZHANG Xiao, LI Chao, WANG Hua, MAI Ai-Guang, YAN Hua, HUANG Hai, FAN Shi-Cai
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  284-287.  doi:10.13418/j.issn.1001-165x.201
    Abstract ( 596 )  
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    Objective To study the methods of digital measurement of the morphology of posterior proximal tibia and to investigate the significance in the treatment of tibial plateau fractures. Methods   Tomographic images of 60 healthy human tibial specimens were obtained by CT scans and imported into the Mimics 10.01 software for 3D reconstruction. Then the related anatomical parameters were measured and analyzed. Results The posterior central height was (8.46±1.62)mm, the posteromedial height was (12.27±1.93)mm, the posteromedial slope length was (14.71±2.27)mm, the posteromedial plateau-slope angle was (132.02±11.62)°, the posteromedial slope-diaphysis angle was (147.28±10.72)°, the posterolateral height was (11.31±1.74)mm, the posterolateral height was (14.45±2.26)mm, the posterolateral plateau-slope angle was (124.01±9.81)° and the posterolateral slope-diaphysis angle was (141.88±9.09)°. There were no statistically significant differences between different sides(P>0.05). The Posterior central height, the posterolateral height and the posterolateral height were correlated to the total length of tibia(P<0.01). Conclusion The three-dimensional reconstruction model has a high fidelity and a high accuracy in anatomical measurement. It can provide anatomical references in the clinical managements and implants designing of posterior tibial plateau fractures.

    Anatomical observation and MRI analysis of the spring ligament
    YU Yong-Xin, LIU Kai, SHAO Gong-Yan, CHANG Ru-Guo
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  288-291.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 812 )  
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    Objective To observe the anatomical characteristics and explore the function of the spring ligament by anatomical study, and to observe morphology of the spring ligament in MRI images. Methods The spring ligament was dissected; its origin, distribution and morphological characteristics were recorded. Meanwhile, analysis of the spring ligament in different scanning planes of the MRI images were carried out. Results The spring ligament is made up of the superomedial calcaneonavicular ligament, the medioplantar oblique calcaneonavicular ligament and the inferoplantar longitudinal calcaneonavicular ligament, which originated from the sustentaculum tali of the calcaneus, and attached to the superomedial aspect of the navicular bone,the bottom of the navicular tuberosity and the navicular beak, respectively. The spring ligament could be displayed clearly on MRI, which was a low signal on T1 and T2 weighted images, and it is better displayed on the sagittal and transverse planes. Conclusion Spring ligament is an important complex of ligamentaneous structures in bearing the head of talus, which has a great significance in maintaining the longitudinal arch of the foot. MR can be used as a diagnostic tool when the spring ligament is injured.

    Imaging anatomic study of uncinate process and lacrimal fossa and its role in the endoscopic transnasal dacryocystorhinostomy
    CHEN Hua-Hua, XIE Min-Jiang, WANG Jin-Yu, CA Xiao-Jian, FANG Ting-Song
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  292-295.  doi:10.13418/j.issn.1001-165x.201
    Abstract ( 890 )  
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    Objective To explore the relationship between the uncinate process and the lacrimal fossa, and to provide guidance for the surgical approach of the endoscopic transnasal dacryocystorhinostomy.  Methods The relationship between uncinate process and lacrimal fossa was observed in 80 cases. The thickness of the lacrimal fossa was measured. 18 patients with dacryocystitis were treated with endoscopic transnasal dacryocystorhinostomyduring which process the lacrimal bone was located anterior to the attachment of the medial or low part of the uncinate process to the lateral nasal wall, the lacrimal bone was nibbled away, and the lacrimal sac was then exposed upward. The feasibility was observed. Results The average bony thickness of the anterosuperior part of lacrimal fossa was (2.96±0.33) mm, while the bony thickness of the posteroinferior half was (0.02±0.01)mm(P<0.05). The uncinate process was more frequently located posterior to the lacrimal fossa at the lower level, then adjacent to lacrimal bone or the frontal process of maxillary bone at the intermediate level, last adjacent to the lateral middle turbinate or orbital lamina at the upper level. lacrimal sac were successfully exposed  in endoscopic transnasal dacryocystorhinostomy in 16 cases anterior to the attachment of the uncinate process, in 2 cases after partial removal of uncinate process because the superior part of unicinate process was located too anteriorly. Conclusion Accurately recognizing the anatomic relationship of uncinate process and lacrimal fossa helps to improve the efficiency of surgery and avoid complications.

    Notexin effects on the survival and proliferation of CD8+ T cell in the injuried-muscle draining lymph nodes
    MA Beng, SHU Rong, LIU Nie-Hui, SHI Dan-Dan, CENG Hui-Jun, CAO Biao, LIAO Hua
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  296-299.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 447 )  
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    Objective  To explore the effects of intramuscular injection of Notexin on transferred OT-I cells in muscle draining lymph nodes of C57BL/6 mice. Methods Tibialis anterior(TA)injury were performed by intramuscular injection of Notexin, or crush skill on B6 mice, separately. CFSE-labeled OT-I cells were adoptively transferred to myoinjury mice, and followed by OVA i.m. injection. TA draining lymph nodes were received, and lymphocytes were collected and analyzed the proliferation of OT-I cells by CFSE fluorescence intensity evaluation using flow cytometry. Activated spleen cDC cells were collected and purified from one B6 mouse received OVA i.v. injection. Purified cDC cells were co-cultured with CFSE-labeled OT-I cells. Notexin with different concentration were added into co-culturing system. After 72h, the survival and proliferation of OT-I cells were detected. Results According to the detection results of CFSE fluorescence intensity, OT-I cells in crush induced-damaged muscle draining lymph nodes were activated and proliferated rapidly under the stimulation of OVA antigen. For Notexin injection-induced muscle damage, no cell proliferation of OT-I cells were detected in TA draining lymph nodes at the day 4, but the proliferation of OT-I cells recovered at the day 7. Co-culturing with activated cDCs cells induced a obvious proliferation of OT-I cells in vitro, however, OT-I cells lost the proliferation ability if the co-culturing system were added with Noteixn, even if the concentration of Notexin was very lower(1:1000). Conclusions The activity and proliferation of CD8+ T cells will be interfered by intramuscular injection of Notxin. Therefore, we suggest that Notexin injection is unsuitable for investigating T cell response associated with myoinjury.

    The effect of NF-κB signaling pathway on iNOS expression in BLP-tolerized macrophages
    LI Xue, WANG Xi-Gan, LUO Hai-Hua, ZHONG Yu-Yun, LEI Ye-Ming, LEI Shan, CA Jun-Wei, JIANG Yong, LIU Jing-Hua
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  300-305.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 336 )  
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    Objective In order to explore mechanisms underlying enhanced bacterial clearance of BLP-tolerized macrophages,we detected the expression of inducible nitric oxide synthase (iNOS) and investigated whether this expression was regulated by NF-κB signaling pathway in BLP-tolerized macrophages to bacterial infection. Methods  Through comparison of the phagocytosis and intracellular bacterial killing of E.coli between Naive and BLP-tolerized mice bone marrow-derived macrophages (BMMs), we evaluated the bacterial clearing capability of BLP-tolerized macrophages. Next, the mRNA level of iNOS in BLP-tolerized macrophages was detected by real-time PCR, and the translocations of p65 from cytoplasm to nucleus were shown by immunofluorescence.Finally, the activation of NF-κB signaling pathway was inhibited and the mRNA expression of iNOS in BLP-tolerized BMMs were observed. Results Compared to Naive macrophages, the phagocytosis and intracellular bacterial killing of BLP-tolerized macrophages were significantly enhanced (P<0.05). The mRNA level of iNOS in BLP-tolerized macrophages was significantly increased (P<0.05), which could be significantly affected when the activation of NF-κB signaling pathway was inhibited (P<0.05). Conclusion The study suggests that iNOS expression increases through the activation of NF-κB signaling pathway in BLP-tolerized macrophages to bacterial infection.

    The role of miRs in the process of promoting myoblast proliferation by mechanical stretch
    ZHANG Ma-Hui, WANG Yong-Kuai, JIANG Xin-Lu, TU Lei, OU Yang-Jun, QIU Xiao-Zhong, WANG Le-Yu
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  306-311.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 344 )  
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    Objective To explore the role of three kinds of miRs in the process of promoting myoblast proliferation by mechanical stretch. Methods Application of cyclic mechanical strain using the computer-controlled Flexcell Strain Unit cultured C2C12 cells. CCK-8 assay was applied to test myoblast proliferation in different conditions of mechanical stretch. The expression profiles of miRs in mechanical stretch groups and control groups were detected by high-throughput sequencing. The results were verified by reverse transcription quantitative PCR(qRT-PCR) and analyzed by bioinformatics. Results C2C12 cells in 0.125Hz stretching group was significantly observed with promoted proliferation of C2C12 myoblasts(P<0.05), but no significant difference of cell proliferation were detected inthe 0.25 Hz and 0.5Hz groups compared to that in the control group; Using  high-throughput sequencing technology, we found 11 kinds of miRs which  were co-expressed and differentially expressed in the two groups (Control group and 0.125 Hz stretching group); the difference was statistically significant in the expression of three miRs: mir-44 , mir-36 and mir-20. These three miRs expression was verified by qRT-PCR, and the results were consistent with the sequencing. These three miRs were involved in several signaling pathways. Conclusions High-throughput genome sequencing and bioinformatics analysis show that three kinds of miRs play an important role in the proliferation of C2C12 myoblast.

    Changes of MCT2 expression in cerebral cortex after formalin-induced rat pain model
    SONG Kai-Qin, KANG Cheng-Qiao, SUN Shan-Quan, HUANG Juan, XU Jin, LI Wen-Juan, JIANG Jin
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  312-315.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 357 )  
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    Objective To investigate the changeofmonocarboxylate transporters 2( MCT2) expression in cerebral cortex primary somatosensory cortex hindlimb region(S1HL)under the condition of pain stimulation, so as to explore the involvement of MCT2 in the pain adjustment mechanism in a rat pain model.    Methods    Immunohistochemical staining(IHC), Western blot and Computing-image analysis system were used to detect the changes of MCT2 expression in the cerebral cortex S1HL of the formalin-induced rat pain model.    Results    Compared with the control group, the number and IOD of MCT2-positive neurons in the cerebral cortex S1HL of formalin-treated rats began to increase at 1h,reaching the highest level on 3d and declined on 7d ,but still higher than those in the control group (P<0.05).The Western blot showed that the alteration tendency of MCT2 expression was consistent with those of the number and IOD of MCT2-positive neurons.    Conclusion    Our results indicated that MCT2 is up-regulated under the condition of pain stimulation, which might be involved in the formalin-induced pain and behavior regulation.

    The protective effect of chlorogenic acid on Aβ25-35-induced damage to cerebral cortical neurons via macrophage activation
    ZHOU Jing, DIAO Hong, QIN Shu-Jian, TAO Su-Yan, ZHENG De-Yu
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  316-320.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 325 )  
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    Objective To investigate the effect of chlorogenic acid on cerebral cortical neurons injury induced by amyloid β protein via macrophage activation in vitro. Methods Cerebral cortical neurons were obtained from the cerebellar cortex of Sprague-Dawley (SD) rats and cultured. At the day 8 of culture, peritoneal macrophage of mice were added to the cerebral cortical neurons cultures and continued to be cultured for additional 2 days. Thereafter, Aβ25-35 (10 μmol/L) 10 μl were added into the co-culture of cerebral cortical neurons and macrophages. The experiment was divided into blank isolated culture group, co-culture group,  isolated culture Aβ25-35 group, co-culture Aβ25-35 group and co-culture CHA-treated group for 6, 12, 24, 48, 96h,   respectively. The survival of neurons and macrophages was detected by an inverted phase contrast microscope. Expressions of p38MAPK, MAPKAPK-2, HSP27 protein were tested by Western blot. Results Application of chlorogenic acid significantly improved the morphology and survival of cerebral cortical neurons damaged by Aβ25-35-induced macrophage activation.  In macrophages, the Aβ25-35-induced p38MAPK pathway activation was inhibited by CHA at different time points. Accordingly, both the decreased expressions of phosphorylated MAPKAPK-2 and phosphorylated HSP-27 in macrophages caused by Aβ25-35 exposure were suppressed by CHA. Conclusion Chlorogenic acid is effective in reducing Aβ25-35-induced damage to cerebral cortical neurons co-cultured with macrophages. Its protective effect may be in part achieved by action  against the upregulation of p38MAPK expression in macrophages.

    The study of effects of low frequency electromagnetic field on the regeneration of hair follicles and expression of KGF in the anagen phase
    WANG Xin, LI Xin-Beng, XIE Yan, BAI Li-Meng, BAI Wen-Fang, SHU Hong-Xiang, HU Wei-Cheng, ZHANG Ming-Sheng
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  321-324.  doi:10.13418/j.issn.1001-165x.201
    Abstract ( 439 )  
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    Objective To observe the effects of low frequency electromagnetic field on the growth of hair follicles and expression of keratinocyte growth factor in the anagen of the hair follicle. Methods The female C57BL/6 adult mice were selected to make the model of the anagen of the hair follicle, and randomly divided into the control group and the low frequency electromagnetic field group which was exposed to 50Hz、5mT electromagnetic field,30min/d, for 16d.After being exposed to the low frequency electromagnetic field for D3 , D9 and D16, the full-thickness skin of the hair removal site was used to detect the changes of morphology of the hair follicle and expression of keratinocyte growth factor in the anagen of the hair follicle. Results    Hematoxylin-eosin staining results showed that: at 3 d,Hair bulb partially surrounded the dermal papilla, and there was melanin above the dermal papilla in the low frequency electromagnetic field group ;at D 9 and D16,the length of the regeneration of hair follicle in the low frequency electromagnetic field group was increased. Fluorogenic quantitative PCR and Western-Blot analysis showed that the gene and protein expression of keratinocyte growth factor in the low frequency electromagnetic field group was significantly up-regulated compared with the control group (P<0.05). Conclusions The result showed that the low frequency electromagnetic field may promote the secretion of keratinocyte growth factor to advance the growth of hair follicles in the anagen of the hair follice.

    PNGF conduit seeded with bone marrow derived mesenchymal stem cells to repair 12-mm sciatic nerve defect of rat
    ZHANG Wei-Cai, HUANG Ji-Feng, YAN Qiong-Jiao, LI Shi-Pu
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  325-329.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 458 )  
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    Objective To evaluate the effectiveness of tissue-engineered artificial nerve consists of novel arginine-glycine-aspatic acid (RGD) peptide modification of poly{(lactic acid)-co-[(glycolic acid)-alt-(L-lysine)]}/poly(d,l-lactic acid)/β-tricalcium phosphate/nerve growth factor(PRGD/PDLLA/β-TCP/NGF,PNGF) sustaining release conduit seeded with Bone marrow derived mesenchymal stem cells(BMSCs) in bridging 12-mm sciatic nerve defect in rats. Methods 30 isogenic adult male Wister rats were randomly divided into 3 groups (n=10 in each), the left sciatic nerve was exposed to create a 12-mm gap in all rats. Animals in the three groups were bridged with PNGF conduits (A), PNGF conduits filled with BMSCs(B) and autografts, respectively. The left was the experimental side and the right was the normal, intact control. At three months after the operation, recovery rate of wet weight of triceps suraes, histological observation of regenerative nerve and target muscle tissue were performed to evaluate the functional recovery of sciatic nerve. Results The conduit wall became thinner three months after the operation, continuously regenerated nerve tissue was located in the conduit, and the surface of regenerated nerve can be observed with creeping growth of nutrient vessels. All regenerated nerves were thinner than normal, intact nerves. The result of SFI in Group B was superior to that of Group A, and the difference was statistically significant(P<0.05);  the recovery rate of nerve conduction velocity, the recovery rate of weight triceps surae, the number of myelinated nerve fibers and thickness of myelin sheath in Group B were significantly higher than those in Group A(P<0.01), acquiring similar results to that of autologous nerve transplantation group.   Conclusion    PRGD/PDLLA/β-TCP/NGF composite nerve conduit seeded with Bone marrow derived mesenchymal stem cells can effectively promote nerve regeneration after bridging sciatic nerve defect in rats. The effect was as good as that of autograft.

    The regeneration of an acellular ureter transplanted to the bladder
    SHAO Ying-Kuan, LIN Ke-Zhi, TU Ya-Ling, RAO Zhi-Heng, LI Re-Bing, MEI Jin
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  330-334.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 650 )  
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    Objective After decellularization,  a rat’s ureter attached to a piece of bladder wall was transplanted to another rat’s bladder wall. Without reseeding any cells, the body itself was to reconstruct the ureter. Methods 20 SD rats’ ureter were decellularized by Triton-X100 and SDS followed by sterilization, after authentication of the structure of the scaffolds we transplanted it onto another rat’s bladder wall with ligation of the distal part of the ureter. After 1-4 weeks, we sacrificed those rats and processed the transplanted structures with HE stain and immunofluorescence for observation and analysis.    Results  We divided the ureters into three parts the part attached to the bladder(B)、the middle part(M)、the freeend (D). After 4 weeks , the epithelial cells and smooth muscles cells could be found in B part, fat cells in M part and fibrosis in D part. Particularly, after 1-2weeks, there are epithelial cells regrown in B part and muscle cells regrown after 4 weeks. Conclusion It is possible that ureter scaffolds can regenerate in body without reseeding and the epithelial layer would regrow first before muscular layer .

    The experimental research of allogeneic bone and periosteum for the treatment of New Zealand white rabbit bone defects
    BI Zhi-Wei, HUANG Dong, ZHANG Da-Wei, NIU Guo-Qiang, OU Yang-Hai-Xiang, LI Yu-Meng
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  335-339.  doi:10.13418/j.issn.1001-165x.201
    Abstract ( 392 )  
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    Objective  To investigate the feasibility and repair mechanism of periosteum and allogeneic bone for treatment of bone defects in the New Zealand white rabbits. Methods 48 New Zealand white rabbits were randomly divided into 3 groups, namely, the experimental group, control group and blank group. The effect and mechanism of periosteum and allogeneic bone harvested for treatment of bone defects was determined by X-ray, real time fluorescent quantitative PCR and HE staining. Results The bone defects of the experimental group and control group healed while the bone defects of the blank group did not heal as shown by the examination of X-ray. The new bone trabecular arrangement was good in the experimental group and there was still a small amount of residual bone allograft according to HE staining. The new bone trabecular arrangement was good in the control group too. The blank group could not see clear bone trabeculae. The Spp-1 and BMP-2 gene expression of the experimental group was higher than that of the control group (P<0.05).  The ColⅠα1 gene expression of the experimental group was lower than that of the control group (P<0.05). Conclusion Periosteum and allogeneic bone could effectively promote the healing of bone defects.

    Pathological features of urethral materials for urethroplasty
    XIE Qi-Gen, SU Cheng, LI Zuo-Jing, XU Zhe, SUN Dun-Jie, ZHOU Li
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  340-342.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 558 )  
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    Objective To observe the pathological features of urethral materials for urethroplasty and provide guidance for the selection of urethroplasty materials. Methods From September 2011 to October 2013 in Pediatric Surgery of the First Affiliated Hospital of Sun Yat-sen University, 20 cases of proximal hypospadias were corrected for ventral curvature. During the operation, specimen including prepuce, ventral penile skin, scrotal septal skin and scrotal skin were collected, fixed and embedded in paraffin. Then tissues were cut into thin slices, followed by Masson staining and observation under the microscope. 5 HPF were photographed randomly by blinded researchers, then the density of hair follicles, sebaceous glands and vessel as well as the thickness of the skin were measured and calculated. Results Comparison of the density of hair follicles and sebaceous glands: scrotal septal skin < scrotal skin (P<0.05), while no hair follicles and sebaceous glands were found in the prepuce and ventral penile skin. Comparison of vessel density: prepuce > ventral penile skin > scrotal septal skin > scrotal skin (P<0.05). Comparison of skin thickness: prepuce < scrotal skin < ventral penile skin < scrotal septal skin (P<0.05). Conclusions Prepuce and ventral penis skin are ideal candidates for urethroplasty, while the scrotal septal skin can be utilized when the Urethral material is insufficient. Scrotal skin is generally not recommended in urethroplasty.

    The finite element analysis of the total hip replacement with cup invagination in dysplasia of hip
    HU Jie, MA Re-Fan, CA Zhi-Qing, LI De, DING Yue, LIU Chang-Li
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  343-347.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 508 )  
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    Objective Using the three dimensional finite element analysis, the stress distribution in acetabular cup-bone interface in different ranges of cup invagination during total hip replacement (THR) in treating the dysplasia of hip affected was studied. Methods Pelvis  in the dysplasia was scanned by CT. Using the computer simulation technology to reconstruct the three-dimensional model of the pelvis for observing the dysplasia of hip from CT scan images. Different ranges of cup invagination in THR in the pelvic model were simulated. Then the pelvis and acetabular cup model were meshed. the mechanics analysis tool in SolidWorks was used to analyze the three-dimensional model, and the stress data in acetabular cup-bone interface was obtained. After the statistical analysis of the experiment result, the different ranges of cup invagination that affected the stress distribution in acetabular cup-bone interface were observed.   Results greater contact area and smaller stress per unit area were resulted due to acetabular deepened grinding and cup invagination. With the increasing degree of acetabular deepened grinding, the bone in the acetabular wall broke more obviously, leading to increasing the stress concentration at the top of the acetabulum and uneven stress in the rest. Conclusion Acetabular deepened grinding and cup invagination improve bone bed inclusion of cup during total hip replacement in treating the dysplasia of hip. However, the increasing deepened grinding and perforation of acetabular wall will lead to significant changes of the stress distribution of acetabula and stress concentration. The perforation of acetabular wall should be minimized.

    Sesamoidbone appear beside the malleolus ulnaris of wrist joints:case report
    TIAN Shu, ZHANG Yang-Ze, CHEN Wei
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  347.  doi:10.13418/j.issn.1001-165x.201
    Abstract ( 711 )  
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    Biomechanical evaluation of stability of implantation of transoralatlantoaxial lateral mass fusion cage
    ZHANG Hao-Na, YIN Qiang-Shui, LIU Gui-Yang, YANG Chao, HUANG Wen-Hua
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  348-350.  doi:10.13418/j.issn.1001-165x.201
    Abstract ( 473 )  
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    Objective To evaluatethe biomechanical properties of the fusion cage combined with Type ⅢTransoralAtlantoaxial Reduction Plate(TARP) system and posterior C1-C2 pedicle screw-rods system. Methods 6 fresh-frozen human cervical spines were used for study in five different groups. Range of motion (ROM) in flexion-extension, left-right bending, and axial rotation was recorded by Motion Analysis, co.6 Eagle.    Results According to the anatomic data, the ring shape of the cage was feasible and the parameters: 3.5 mm height, 6 mm inner diameter, and 12mmouter diameter. The cage was made of Poly Ether EtherKetone(peek).The difference in stiffness between the TARP and C1-C2screw-rods was not statistically significant in the same implant groups. The ROM of cage groups was smaller compared with ilium groups and the difference in flexion-extension was statistically significant. Conclusions TARP system fixation or pedicle screw-rods fixation was significantly stiffer than the iliumgroup in atlantoaxial fusion. Moreover, smaller postoperative complications render it to have a better prospect of clinical application in clinical practice.

    The clinical use and significance of the spatial anatomical separation of myopectineal orifice in totally extraperitoneal prosthesis for repair of inguinal hernia
    HUA Wei, LIANG Zhi-Hong, DIAO Xiang-Wen, DING Ji-Wei, SHANG Chi-Beng, DING Zi-Hai
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  351-353.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 405 )  
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    Objective To investigate the significance of using laparoscope technology to identify the spatial anatomy of myopectineal orifice in totally extraperitoneal prosthesis for inguinal hernia. Methods Data of 272 TEP cases from 2005 to 2013 was analyzed retrospectively. The myopectineal orifice was divided into 4 spaces during the operation, and the 4 spaces were anatomically separated one by one. Results Proper anatomical landmarks were used to divide the myopectineal orifice into four spaces, and the four spaces finally formed a larger space. All the cases were operated using the technique of spatial anatomical separation of myopectineal orifice. The mean surgical length was 48 minutes (28~60), and the mean postoperative hospitalization length  was 5 days(3~8). 2 cases had scrotal seroma after the operation, which were both cured with suction. No recurrence, infection, chronic pain, testicular atrophy and ejaculation pain appeared.   Conclusions    The technique of spatial anatomical separation of myopectineal orifice shortened the learning time of mastering TEP,  deepened the understanding ofthe complicated anatomy of myopectineal orifice and the structure behind it, which can lead to more efficient operation and fewer surgical complications.

    The study on the correlation between the relative height of the humeral head and the subacromial impingement syndrome
    BO Zhao-Xun, CUI Yan, SUN Chao, MA Hai-Meng, ZHANG Hong-Xin
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  354-356.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 652 )  
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    Objective To provide new theoretical basis for clinical diagnosis and treatment of subacromial impingement syndrome. Methods Using MSCT and PC software we measured the relative height of the humeral heads in 26 cases who sustained subacromial impingement syndrome and 26 cases of healthy adults who were randomly selected. Comparison of the relative height of the humeral heads in two groups was then carried out. Results The measurement results of the relative height of the humeral heads was (0.88±0.18) cm in the experimental group and was (1.07±0.20)cm in the control group. Compared with the control group, the relative height of the humeral heads in the experimental group was smaller (P<0.01) Conclusion The risk of development of subacromial impingement increases with a larger relative height of the humeral heads and vice versa.

    The clinical curative effect of distal radius fracture in the elderly by the minimally invasive locking compression plate (LCP)
    TUN Feng, XIE Jin, MO Mao, JU Zhong-Bing
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  357-360.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 311 )  
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    Objective To investigate the clinical curative effect of distal radius fracture in the elderly by the minimally invasive locking compression plate.    Methods   30  cases of distal radius fracture in the elderly treated by LCP using the minimally invasive technique through the volar approach in our hospital from January 2008 to January 2013 were retrospectively analyzed.  According to the AO/ASIF classification: there were 3 cases of A2 type, 5 cases of A3 type, 5 cases of  B1 type, 6 cases of B3 type, 6 cases of C1 type and 5 cases of C2 type.   Results    25 patients were followed up for an average of 12 months (8~14 months) postoperatively. It turned out 1 case developed the carpal tunnel syndrome,1 case developed thenar eminencet myoparalysis, 1 case developed delayed healing of the wrist incision.  postoperative symptoms disappeared in 3 months. No non-union of the radius fracture was found. According to Mcbride’s modified evaluation criteria: 18 cases were excellent,4 cases good,3 cases common, leading to a rate of excellence of being 88%. Conclusion    LCP treatment of the distal radius fracture in the elderly by minimally invasive through volar approach is mild in trauma  firm in fixation, and rare in complications. This method enables early functional exercise, making it a more ideal fixed method.

    The diagnostic value of transbronchial lung biopsy and CT-guided percutaneous lung puncture for occupying lesions in the lung space
    CUI Hai-Yan, LIANG Zhen-Yu, ZHANG Da-Cheng, QUAN Guo-Chi, CENG Cong
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  361-364.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 509 )  
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    Objective To evaluate the diagnostic value of transbronchial lung biopsy and CT-guide percutaneous lung puncture for occupying leslies in the lung space. Methods 192 patients with occupying lesions in the lung space were selected and All received chest radiograph and chest CT. 126 of them underwent transbronchial lung biopsy and 66 patients underwent lung biopsy through CT guidedpercutaneous lung punctureThe diagnostic rate of two methods were compared to evaluate the diagnostic value for occupying lesions in the lung space. Results The size and shape of occupying lesions in the lung space can be directly observed by bronchofiberscope. The diagnostic rate of occupying lesions in the lung space by transbronchial lung biopsy and CT-guided percutaneous lung puncture were 83.1% and 88.7%. There was no significant difference between two methods for the diagnosis of occupying lesions in the lung space. However, compared to CT-guided percutaneous lung puncture, transbronchial lung biopsy had lower incidence of complication and higher safety with statistical significance (P<0.05). Conclusion The diagnostic value of transbronchial lung biopsy and CT-guided percutaneous lung puncture for occupying lesions in the lung space is similar. But compared to CT-guided percutaneous lung puncture, transbronchial lung biopsy is safer.

    Effect of postoperative analgesia with ultrasound guided femoral nerve block used in arthroscopically-assisted knee operation
    ZHANG Jian-Xing, DENG Lian, WANG Xing, LI Gong-Yang
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  365-367.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 262 )  
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    Objective To research the effect of postoperative analgesia with ultrasound guided femoral nerve block used in arthroscopically-assisted knee operation.  Method Sixty patients undergoing arthroscopically-assisted knee operation were divided equally and randomly into two groups: normal group (A, n=30) and research group(B, n=30). All of the patients were anesthetized with spinal anesthesia. Patients in group B received ultrasound-guided femoral nerve block, which patients in group A received no further management. the analgesic effect(VAS) 6h、12h、24h postoperatively and analgesia drugs consumption, incidence of side effects, degree of comfort in 24 hours postoperatively in all patients were observed.  Results  Compared with group A,analgesic effect was significant better with less extra drugs consumption and more comfort in group B. Group B had incidence of complications but no statistical difference could be detected.  Conclusion Ultrasound guided femoral nerve block used in arthroscopically-assisted knee operation can bring better postoperative analgesia and reduce the adverse effects. It can make patients  feel comfortable.

    Degloving injury of the thumb treatment progress
    SONG Er-Fa, JU Ji-Hui, HOU Rui-Xin
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  368-370.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 376 )  
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    Factors affecting cartilage differentiation of BMSCs
    FANG Chu-Ling, TIAN Jing
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  371-374.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 506 )  
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    A case of anterior communicating aneurysm in a patient with right aortic arch 
    LIU Pan-Chao, DUAN Chuan-Zhi, LI Xi-Feng, HE Xu-Yang, ZHANG Xin
    Chinese Journal Of Clinical Anatomy. 2014, 32(3):  375.  doi:10.13418/j.issn.1001-165x.2014
    Abstract ( 382 )  
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