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Effect and mechanism of plumbagin on reducing podocyte injury in rats with IgA nephropathy by regulating ROS and inhibiting NLRP3
- LIU Chang-ming, HUANG Xiang-chen, DU Bin
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Chinese Journal of Clinical Anatomy. 2020, 38(3):
308-313.
doi:10.13418/j.issn.1001-165x.2020.03.013
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Abstract
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329 )
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Objective To investigate the role and mechanism of plumbagin in IgA nephropathy. Methods First, the IgA nephropathy model was constructed according to the modified BSA+LPS+CCl4 method of Ying et al. Then, the rats in the administration group were intraperitoneally injected with 10 mg/kg, 20 mg/kg or 50 mg/kg of plumbagin per day. The normal group and the model group were intraperitoneally injected with the same amount of normal saline per day, after 8 weeks later, 24 h urine protein, serum creatinine, and blood urea were detected by a fully automatic chemical analyzer, and the ROS content was detected by flow, and the SOD activity and MDA content were detected. Pathological damage was observed by HE staining, TNF-a, IL-18, IL-1β were detected by Elisa, and NLRP3, ASC, caspase-1 p20, P13K, protein expression of AKT and NF-kB were detected by Western blotting. Results Compared with the model group, the proteinuria, serum creatinine and urea nitrogen levels of the administration group significantly reduced, ROS levels significantly decreased, SOD levels increased, MDA, IL-1b, IL-18 and TNF-a levels were significantly increased. Protein expression of NLRP3, ASC, caspase-1 p20, P13K, AKT and NF-kB was significantly down-regulated, with the increasing of dose, the effect was more pronounced. Conclusions Plumbagin mainly reduces IgA nephropathy by reducing urinary protein, serum creatinine and blood urea, reducing pathological damage, inhibiting oxidative stress, inflammatory response and activation of NLRP3 / P13K / AKT / NF-kB pathway.
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