Objective To provide variation data of pulmonary fissure for imaging diagnosis. Methods 451 patients without obvious lesions and operation history were performed a 64-slice multidetector CT scan with the thickness of 0.6 mm, and then the raw data were reformed at the sagital and coronal orientation with the slice thickness of 3mm. All of the reformed images were observed and the features of the pulmonary fissure were counted and analyzed. Results Variations of pulmonary fissures appeared in 312 cases (69.18%), among them, 282 cases (62.53%) were right variation, in which 177 cases were single variation, 105 were complex variation. While 82 cases (18.18%) were left variation, in which 25 cases were single and 57 were complex. Three types of variations were observed: type 1, incomplete pulmonary fissures, 45.45% in right horizontal fissure and 19.51% in right oblique fissure, and 16.41% in left oblique fissure; type 2, pulmonary fissure absence, 0.89% in the right horizontal fissure, 0.22% in the right oblique fissure and the horizontal fissure, 0.44% in the left oblique fissure; type 3, accessory fissure, with the occurrence rate of 13.97%, 11.97% in the right, 2.44% in the left. Conclusions It is an efficient method to observe and classify the variations of the pulmonary fissures adopting 64-slice MSCT scan combined with MPR technique, which will provide reliable and useful imaging information for correlative subjects.